Busca avançada
Ano de início
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of Laser Therapy on Immune Cells Infiltrate After Excisional Wounds in Diabetic Rats

Texto completo
Santana, Cristiano de Loura [1] ; Teixeira Silva, Daniela de Fatima [1] ; de Souza, Amanda Pires [1] ; Jacinto, Marcos Vinicius [1] ; Bussadori, Sandra Kalil [1] ; Mesquita-Ferrari, Raquel Agnelli [1] ; Santos Fernandes, Kristianne Porta [1] ; Franca, Cristiane Miranda [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Nove Julho, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Lasers in Surgery and Medicine; v. 48, n. 1, p. 45-51, JAN 2016.
Citações Web of Science: 4

Background and Objective: Diabetes alters innate and specific immunity, causing an imbalanced tissue repair process. Very active neutrophils and macrophages are found for a long time in chronic wounds in those individuals. The aim of this study was to evaluate the response of the main effector cells of immunity (neutrophils, macrophages, and T lymphocytes) and to compare the effects of two laser therapy regimens in the postoperative treatment of excision wounds. Method: Diabetes was induced in female Wistar rats and a punch was used to cause wounds in the dorsum of each individual. The animals were randomly allocated to a control group (CG), in which the wound was untreated, a single-dose laser group (SLG), in which the wound was submitted to single dose of laser therapy at wavelength of 660 nm, output power of 30mW, energy density of 4 J/cm(2), and 26-second exposure time, and a fractionated-dose laser group (FLG), submitted to 1 J/cm(2) of laser therapy on Days 1, 3, 8, and 10. Euthanasia was performed on five animals from each group Days 1, 3, 8, 10, 15, and 22. The wound was removed and routinely processed for immunohistochemistry against elastase, CD3, CD68, and CD206 antibodies. The samples were photographed and labeled cells were counted by a blinded observer. The Kruskal-Wallis test was used for the statistical analysis. Results: Neutrophils were predominant in the SLG on Day 1, whereas these cells were mostly found in the CG on Day 3 (P<0.05). The T lymphocyte count was similar in all groups in the throughout the experiment. On Day 3, the SLG exhibited a greater number of total macrophages (CD68+) (P<0.05), whereas the macrophage count was similar among the different groups on the other evaluation days. The CD206+ cell counts revealed that the SLG had more M2 macrophages than the CG on Day 8 (P<0.05), whereas the FLG exhibited more M2 macrophages than the CG on Day 10 (P<0.05). Conclusion: The present findings demonstrate that laser therapy can alter the composition of inflammatory infiltrate in diabetic wounds, leading to a more balanced response transiting from a rapid neutrophil infiltration through to M2 macrophage polarization, especially with a single application of 4 J/cm(2) in the immediate postoperative period. (C) 2015 Wiley Periodicals, Inc. (AU)

Processo FAPESP: 12/01944-0 - Estudo da ativação celular e de citocinas no reparo cutâneo de ratos diabéticos tratados com laser em baixa intensidade
Beneficiário:Cristiane Miranda Franca
Modalidade de apoio: Auxílio à Pesquisa - Regular