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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rhenium(I) Polypyridine Complexes as Luminescence-Based Sensors for the BSA Protein

Texto completo
Autor(es):
Frin, Karina P. M. [1] ; Nascimento, Veronica M. [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Fed Abc, Av Estados 5001, BR-09210170 Santo Andre, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of the Brazilian Chemical Society; v. 27, n. 1, p. 179-185, JAN 2016.
Citações Web of Science: 2
Resumo

The binding interaction of rhenium(I) complexes fac-[Re(CO)3(NN)(py)]+, py = pyridine and NN = 1,10-phenanthroline (phen), 4,7-diphenyl-1,10-phenanthroline (ph2phen) or 4,7-dichloro-1,10-phenanthroline (Cl2phen), and bovine serum albumin (BSA) was investigated at physiological pH using emission intensity variation and circular dichroism (CD) spectroscopy. The photophysical investigations showed that in the presence of BSA, the metal-to-ligand-charge transfer (3MLCT) emission of the rhenium(I) complexes was quenched due to entrapment of the complex within the protein environment. Additionally, high Stern-Volmer (KSV) and binding (Kb) constants were determined from luminescence data, revealing the occurrence of a strong interaction and/or association. The differences in KSV values can be tentatively associated with an electron-withdrawing constant (σ) defined by Hammett equation. The CD results showed that the extent of α-helicity of the BSA decreased upon the addition of rhenium complexes, which provided further support for the interaction of rhenium(I) complexes and the protein. (AU)

Processo FAPESP: 11/23408-0 - Síntese, caracterização e investigação das propriedades fotofísicas de complexos de rênio (I)
Beneficiário:Karina Passalacqua Morelli Frin
Linha de fomento: Auxílio à Pesquisa - Regular