The glycosylated flavonoids vitexin, isovitexin, and quercetrin isolated from Serjania erecta Radlk (Sapindaceae) leaves protect PC12 cells against amyloid-beta(25-35) peptide-induced toxicity

The A beta peptide-mediated toxicity participates in the neuronal death that occurs in Alzheimer's disease. The present study aims to isolate the major compounds of Serjania erecta Radlk leaves and assess whether these compounds protect PC12 cells from A beta(25-35) peptide-induced toxicity. We isolated three flavonoid glycosides with high purity: quercetrin, vitexin, and isovitexin. The A beta(25-35) peptide alone decreased the PC12 cell viability in a concentration-dependent manner, as evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MIT) assay. We selected the A beta(25-35) peptide concentration of 50 mu M for the experiments. Treatment of PC12 cells with the flavonoids before exposure to the A beta(25-35) peptide increased cell viability, i.e., these compounds protected the cells against A beta(25-35) peptide-induced toxicity. Vitexin promoted higher protection levels than quercetrin and isovitexin, and reduced the lactate dehydrogenase release and NO production in A beta(25-35) peptide-treated PC12 cells. Therefore, the glycosylated flavonoids that exist in S. erecta leaves, especially vitexin, protect PC12 cells from A beta(25-35) peptide-induced toxicity. (C) 2015 Elsevier Ltd. All rights reserved. (AU)