Effects of maternal vitamin B-6 deficiency and over-supplementation on DNA damage and oxidative stress in rat dams and their offspring

Vitamin B-6 is a cofactor for more than 140 essential enzymes and plays an important role in maternal health and fetal development. The goal of this study was to investigate the effects of maternal vitamin B-6 on DNA damage and oxidative stress status in rat dams and their offspring. Female Wistar rats were randomly assigned to three dietary groups fed a standard diet (control diet), a diet supplemented with 30 mg/kg of vitamin B-6, or a deficient diet (0 mg/kg of vitamin B-6) for 10 weeks before and during mating, pregnancy and lactation. The dams were euthanized at weaning, and their male pups were euthanized either 10 days or 100 days after birth. We found that maternal vitamin B-6 deficiency increased the micronucleus frequency in peripheral blood and bone marrow cells and also increased the concentration of hepatic TBARS (thiobarbituric acid reactive substances) in newborn pups (10 days old). In conclusion, maternal 5- to 6-fold over-supplementation of vitamin B-6 had no adverse effects, however its deficiency may induce chromosomal damage and hepatic lipid peroxidation in the offspring. (C) 2015 Elsevier Ltd. All rights reserved. (AU)