Busca avançada
Ano de início
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of protein undernourishment on the condylar process of the Wistar rat mandible correlation with insulin receptor expression

Texto completo
Cavalli, Marcelo Arthur [1] ; Goncalves, Aline [1] ; Bertaglia Pereira, Joice Naiara [1] ; da Silva, Jodonai Barbosa [1] ; Boldrini, Silvia de Campos [2] ; Liberti, Edson Aparecido [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Surg, Fac Vet Med & Anim Sci, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Funct Anat Appl Clin & Surg, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Applied Oral Science; v. 23, n. 2, p. 135-144, MAR-APR 2015.
Citações Web of Science: 2

The mandible condylar process cartilage (CP) of Wistar rats is a secondary cartilage and acts as a mandibular growth site. This phenomenon depends on adequate proteins intake and hormone actions, including insulin. Objectives: The present study evaluated the morphological aspects and the expression of the insulin receptor (IR) in the cartilage of the condylar process (CP) of rats subjected to protein undernourishment. Material and Methods: The nourished group received a 20% casein diet, while the undernourished group (U) received a 5% casein diet. The re-nourished groups, R and RR, were used to assess the effects of re-nutrition during puberty and adulthood, respectively. CPs were processed and stained with picro-sirius red, safranin-O and azocarmine. Scanning electron microscopy and immunohistochemistry were also performed. Results: The area of the CP cartilage and the number of cells in the chondroblastic layer decreased in the U group, as did the thickness of the CP layer in the joint and hypertrophic layer. Renourishment during the pubertal stage, but not during the adult phase, restored these parameters. The cell number was restored when re-nutrition occurred in the pubertal stage, but not in the adult phase. The extracellular matrix also decreased in the U group, but was restored by re-nutrition during the pubertal stage and further increased in the adult phase. IR expression was observed in all CPs, being higher in the chondroblastic and hypertrophic cartilage layers. The lowest expression was found in the U and RR groups. Conclusions: Protein malnutrition altered the cellularity, the area, and the fibrous cartilage complex, as well as the expression of the IRs. (AU)

Processo FAPESP: 06/56045-9 - Efeito da desnutricao proteica pre e pos-natal e da renutricao pos-natal sobre o crescimento craniofacial de ratos wistar nas fases puberal e adulta.
Beneficiário:Silvia de Campos Boldrini
Linha de fomento: Auxílio à Pesquisa - Regular