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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antimicrobial photodynamic therapy against pathogenic bacterial suspensions and biofilms using chloro-aluminum phthalocyanine encapsulated in nanoemulsions

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Autor(es):
Dias Ribeiro, Ana Paula [1] ; Andrade, Mariana Carvalho [2] ; Bagnato, Vanderlei Salvador [3] ; Vergani, Carlos Eduardo [2] ; Primo, Fernando Lucas [4] ; Tedesco, Antnio Claudio [2, 4] ; Pavarina, Ana Claudia [5]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Brasilia UnB, Sch Hlth Sci, Dept Dent, Campus Darcy Ribeiro, DF - Brazil
[2] Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Photobiol & Photomed Res Grp, Ctr Nanotechnol & Tissue Engineers, FFCLRP, Ribeirao Preto, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, BR-14801903 Araraquara, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Lasers in Medical Science; v. 30, n. 2, p. 549-559, FEB 2015.
Citações Web of Science: 13
Resumo

Antimicrobial photodynamic therapy represents an alternative method of killing resistant pathogens. Efforts have been made to develop delivery systems for hydrophobic drugs to improve the photokilling. This study evaluated the photodynamic effect of chloro-aluminum phthalocyanine (ClAlPc) encapsulated in nanoemulsions (NE) on methicillin-susceptible and methicillin-resistant Staphylococcus aureus suspensions and biofilms. Suspensions and biofilms were treated with different delivery systems containing ClAlPc. After the pre-incubation period, the drug was washed-out and irradiation was performed with LED source (660 +/- 3 nm). Negative control samples were not exposed to ClAlPc or light. For the suspensions, colonies were counted (colony-forming units per milliliter (CFU/mL)). The metabolic activity of S. aureus suspensions and biofilms were evaluated by the XTT assay. The efficiency was dependent on the delivery system, superficial load and light dose. Cationic NE-ClAlPc and free-ClAlPc caused photokilling of the both strains of S. aureus. For biofilms, cationic NE-ClAlPc reduced cell metabolism by 80 and 73 % of susceptible and resistant strains, respectively. Although anionic NE-ClAlPc caused a significant CFU/ml reduction for MSSA and MRSA, it was not capable of reducing MRSA biofilm metabolism. This therapy may represent an alternative treatment for eradicating resistant strains. (AU)

Processo FAPESP: 10/05425-1 - Avaliação da efetividade da terapia fotodinâmica antimicrobiana na inativação de espécies de Candida e do dano celular causado a células epiteliais: avaliação in vitro e in vivo da cloro alumínio ftalocianina e e outros derivados
Beneficiário:Ana Cláudia Pavarina
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/17975-9 - Avaliação da efetividade da Terapia Fotodinâmica Antimicrobiana na inativação de espécies de Candida e do dano celular causado a células epiteliais. Avaliação in vitro e in vivo da cloro alumínio Ftalocianina e outros derivados.
Beneficiário:Ana Paula Dias Ribeiro
Linha de fomento: Bolsas no Brasil - Doutorado