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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Validated method for phytohormone quantification in plants

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Trapp, Marilia Almeida [1, 2] ; De Souza, Gezimar D. [3] ; Rodrigues-Filho, Edson [2] ; Boland, William [1] ; Mithoefer, Axel [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Max Planck Inst Chem Ecol, Dept Bioorgan Chem, D-07745 Jena - Germany
[2] Univ Fed Sao Carlos, Dept Chem, LaBioMMi, BR-13560 Sao Carlos, SP - Brazil
[3] Accert Chem & Biotechnol Inc, Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Citações Web of Science: 34

Phytohormones are long time known as important components of signaling cascades in plant development and plant responses to various abiotic and biotic challenges. Quantifications of phytohormone levels in plants are typically carried out using GC or LC-MS/MS systems, due to their high sensitivity, specificity, and the fact that not much sample preparation is needed. However, mass spectrometer-based analyses are often affected by the particular sample type (different matrices), extraction procedure, and experimental setups, i.e., the chromatographic separation system and/or mass spectrometer analyser (Triple-quadrupole, lontrap, TOE, Orbitrap). For these reasons, a validated method is required in order to enable comparison of data that are generated in different laboratories, under different experimental set-ups, and in different matrices. So far, many phytohormone quantification studies were done using either QTRAP or Triple-quadrupole mass spectrometers. None of them was performed under the regime of a fully-validated method. Therefore, we developed and established such validated method for quantification of stress-related phytohormones such as jasmonates, abscisic acid, salicylic acid, IAA, in the model plant Arabidopsis thaliana and the fruit crop Citrus sinensis, using an lontrap mass spectrometer. All parameters recommended by FDA (US Food and Drug Administration) or EMEA (European Medicines Evaluation Agency) for validation of analytical methods were evaluated: sensitivity, selectivity, repeatability and reproducibility (accuracy and precision). (AU)

Processo FAPESP: 12/20932-2 - Estudo sobre o papel ecológico de micro-organismos endofíticos na produção de compostos antibióticos na planta Alternanthera brasiliana
Beneficiário:Marília Almeida Trapp
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado