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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patients

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Taira, Cleison Ledesma [1, 2] ; Okay, Thelma Suely [3] ; Delgado, Artur Figueiredo [4, 5] ; Jurfest Rivero Ceccon, Maria Esther [4] ; Gottardo de Almeida, Margarete Teresa [6] ; Barbaro Del Negro, Gilda Maria [1, 2]
Total Authors: 6
[1] HCFMUSP, Clin Dermartol Div, Lab Med Mycol LIM 53, BR-05403900 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Med Trop, BR-05508 Sao Paulo - Brazil
[3] Univ Sao Paulo, IMT, Lab Seroepidemiol & Immunobiol, Sao Paulo, SP - Brazil
[4] HCFMUSP, Pediat Intens Care Unit, Sao Paulo, SP - Brazil
[5] HCFMUSP, Inst Crianca, Neonatal Intens Care Unit, Sao Paulo, SP - Brazil
[6] FAMERP, Dept Dermatol Infect & Parasit Dis, Microbiol Lab, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: BMC INFECTIOUS DISEASES; v. 14, JUL 21 2014.
Web of Science Citations: 22

Background: Nosocomial candidaemia is associated with high mortality rates in critically ill paediatric patients; thus, the early detection and identification of the infectious agent is crucial for successful medical intervention. The PCR-based techniques have significantly increased the detection of Candida species in bloodstream infections. In this study, a multiplex nested PCR approach was developed for candidaemia detection in neonatal and paediatric intensive care patients. Methods: DNA samples from the blood of 54 neonates and children hospitalised in intensive care units with suspected candidaemia were evaluated by multiplex nested PCR with specific primers designed to identify seven Candida species, and the results were compared with those obtained from blood cultures. Results: The multiplex nested PCR had a detection limit of four Candida genomes/mL of blood for all Candida species. Blood cultures were positive in 14.8% of patients, whereas the multiplex nested PCR was positive in 24.0% of patients, including all culture-positive patients. The results obtained with the molecular technique were available within 24 hours, and the assay was able to identify Candida species with 100% of concordance with blood cultures. Additionally, the multiplex nested PCR detected dual candidaemia in three patients. Conclusions: Our proposed PCR method may represent an effective tool for the detection and identification of Candida species in the context of candidaemia diagnosis in children, showing highly sensitive detection and the ability to identify the major species involved in this infection. (AU)

FAPESP's process: 10/09715-4 - Detection and identification of Candida spp. in bloodstream infections of pediatric patients admitted in intensive care units: validation of a multiplex PCR
Grantee:Gilda Maria Barbaro Del Negro
Support Opportunities: Regular Research Grants
FAPESP's process: 10/02626-6 - Detection and identification of Candida spp. from bloodstream infections of critically ill pediatric patients: validation of a multiplex PCR
Grantee:Cleison Ledesma Taira
Support Opportunities: Scholarships in Brazil - Master