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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Preparation, characterization, and in vitro phototoxic effect of zinc phthalocyanine cucurbit[7]uril complex encapsulated into liposomes

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Bolfarini, Gisele C. [1] ; Siqueira-Moura, Marigilson P. [2] ; Demets, Gregoire J. F. [3] ; Tedesco, Antonio C. [4]
Total Authors: 4
[1] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
[2] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
[3] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
[4] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
Total Affiliations: 4
Document type: Journal article
Source: DYES AND PIGMENTS; v. 100, p. 162-167, JAN 2014.
Web of Science Citations: 14

Inclusion complexes improve the solubility, dissolution, and bioavailability of hydrophobic drugs. In this study, we used the cucurbit{[}7]uril (CB{[}7]) to improve the solubility of zinc phthalocyanine (ZnPc) in water. The CB{[}7]:ZnPc complex was prepared by co-evaporation, characterized it by H-1 NMR spectroscopy, differential scanning calorimetry, X-ray diffraction, UV-vis spectroscopy, and molecular simulation. Next, the CB{[}7]:ZnPc inclusion complex was incorporated into liposomes (CB{[}7]:ZnPc-Lp) to obtain a drug delivery system and assess the in vitro phototoxicity of both free and liposomal CB{[}7]:ZnPc on B16-F10 melanoma cells. H-1 NMR confirmed that the CB{[}7]/ZnPc proportion in the inclusion complex was 1:1, as corroborated by X-ray diffraction, differential scanning calorimetry, and UV-vis spectroscopy. Molecular simulation evidenced that the structures of ZnPc and CB{[}7] interact in the inclusion complex. The thin lipid film method with subsequent extrusion furnished liposomes containing CB{[}7]:ZnPc compared with the free inclusion complex, CB{[}7]:ZnPc-Lp exerted excellent phototoxic effect on melanoma cells. In conclusion, inclusion complexes effectively solubilize hydrophobic compounds, such as zinc phthalocyanine and allow for their incorporation into liposomes, generating excellent drug delivery systems. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/53719-4 - Use of nanocarriers containing photosensitive dyes and other derived and growing factors applied to the photodynamic process in the health areas
Grantee:Antonio Claudio Tedesco
Support type: Research Projects - Thematic Grants