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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation, propagation, genome analysis and epidemiology of HKU1 betacoronaviruses

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Dominguez, Samuel R. [1, 2] ; Shrivastava, Susmita [3] ; Berglund, Andrew [1] ; Oian, Zhaohui [1] ; Bentim Goes, Luiz Gustavo [4, 5] ; Halpin, Rebecca A. [3] ; Fedorova, Nadia [3] ; Ransier, Amy [3] ; Weston, Philip A. [1] ; Durigon, Edison Luiz [4, 5] ; Jerez, Jose Antonio [4, 6] ; Robinson, Christine C. [7] ; Town, Christopher D. [3] ; Holmes, Kathryn V. [2]
Total Authors: 14
[1] Univ Colorado, Sch Med, Dept Pediat, Aurora, CO 80045 - USA
[2] Univ Colorado, Sch Med, Dept Microbiol, Aurora, CO 80045 - USA
[3] Childrens Hosp Colorado, Dept Pathol & Clin Med, Aurora, CO 80045 - USA
[4] J Craig Venter Inst, Rockville, MD 20850 - USA
[5] Univ Sao Paulo, Interdisciplinary Grad Program Biotechnol, BR-05508900 Sao Paulo - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05580900 Sao Paulo - Brazil
[7] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: JOURNAL OF GENERAL VIROLOGY; v. 95, n. 4, p. 836-848, APR 2014.
Web of Science Citations: 6

From 1 January 2009 to 31 May 2013, 15 287 respiratory specimens submitted to the Clinical Virology Laboratory at the Children's Hospital Colorado were tested for human coronavirus RNA by reverse transcription-PCR. Human coronaviruses HKU1, OC43, 229E and NL63 co-circulated during each of the respiratory seasons but with significant year-to-year variability, and cumulatively accounted for 7.4-15.6% of all samples tested during the months of peak activity. A total of 79 (0.5% prevalence) specimens were positive for human betacoronavirus HKU1 RNA. Genotypes HKU1 A and B were both isolated from clinical specimens and propagated on primary human tracheal bronchial epithelial cells cultured at the air liquid interface and were neutralized in vitro by human intravenous immunoglobulin and by polyclonal rabbit antibodies to the spike glycoprotein of HKU1. Phylogenetic analysis of the deduced amino acid sequences of seven full-length genomes of Colorado HKU1 viruses and the spike glycoproteins from four additional HKU1 viruses from Colorado and three from Brazil demonstrated remarkable conservation of these sequences with genotypes circulating in Hong Kong and France. Within genotype A, all but one of the Colorado HKU1 sequences formed a unique subclade defined by three amino acid substitutions (W197F, F613Y and S752F) in the spike glycoprotein and exhibited a unique signature in the acidic tandem repeat in the N-terminal region of the nsp3 subdomain. Elucidating the function of and mechanisms responsible for the formation of these varying tandem repeats will increase our understanding of the replication process and pathogenicity of HKU1 and potentially of other coronaviruses. (AU)

FAPESP's process: 10/00397-0 - Molecular epidemiology of Human Coranavirus HCoV, circulated in São Paulo City from 1995 to 2009
Grantee:Edison Luiz Durigon
Support Opportunities: Regular Research Grants