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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Physiological implications of the regulation of vacuolar H+-ATPase by chloride ions

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Author(s):
L.R. Carraro-Lacroix [1] ; L.M.A. Lessa [2] ; R. Fernandez [3] ; G. Malnic [4]
Total Authors: 4
Affiliation:
[1] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Fisiologia e Biofísica - Brasil
[2] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Fisiologia e Biofísica - Brasil
[3] Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Fisiologia - Brasil
[4] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Fisiologia e Biofísica - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 42, n. 2, p. 155-163, 2009-02-00.
Abstract

Vacuolar H+-ATPase is a large multi-subunit protein that mediates ATP-driven vectorial H+ transport across the membranes. It is widely distributed and present in virtually all eukaryotic cells in intracellular membranes or in the plasma membrane of specialized cells. In subcellular organelles, ATPase is responsible for the acidification of the vesicular interior, which requires an intraorganellar acidic pH to maintain optimal enzyme activity. Control of vacuolar H+-ATPase depends on the potential difference across the membrane in which the proton ATPase is inserted. Since the transport performed by H+-ATPase is electrogenic, translocation of H+-ions across the membranes by the pump creates a lumen-positive voltage in the absence of a neutralizing current, generating an electrochemical potential gradient that limits the activity of H+-ATPase. In many intracellular organelles and cell plasma membranes, this potential difference established by the ATPase gradient is normally dissipated by a parallel and passive Cl- movement, which provides an electric shunt compensating for the positive charge transferred by the pump. The underlying mechanisms for the differences in the requirement for chloride by different tissues have not yet been adequately identified, and there is still some controversy as to the molecular identity of the associated Cl--conducting proteins. Several candidates have been identified: the ClC family members, which may or may not mediate nCl-/H+ exchange, and the cystic fibrosis transmembrane conductance regulator. In this review, we discuss some tissues where the association between H+-ATPase and chloride channels has been demonstrated and plays a relevant physiologic role. (AU)

FAPESP's process: 04/01683-5 - Molecular and functional studies of membrane ion transporters
Grantee:Gerhard Malnic
Support type: Research Projects - Thematic Grants