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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combined immunization using DNA-Sm14 and DNA-Hsp65 increases CD8+memory T cells, reduces chronic pathology and decreases egg viability during Schistosoma mansoni infection

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Espindola, Milena Sobral [1] ; Frantz, Fabiani Gai [1] ; Soares, Luana Silva [1] ; Masson, Ana Paula [2] ; Tefe-Silva, Cristiane [3] ; Bitencourt, Claudia Silva [1] ; Oliveira, Sergio Costa [4] ; Rodrigues, Vanderlei [2] ; Ramos, Simone Gusmao [3] ; Silva, Celio Lopes [2] ; Faccioli, Lucia Helena [1]
Total Authors: 11
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol, BR-14049900 Ribeirao Preto, SP - Brazil
[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BMC INFECTIOUS DISEASES; v. 14, MAY 16 2014.
Web of Science Citations: 5

Background: Schistosomiasis is one of the most important neglected diseases found in developing countries and affects 249 million people worldwide. The development of an efficient vaccination strategy is essential for the control of this disease. Previous work showed partial protection induced by DNA-Sm14 against Schistosoma mansoni infection, whereas DNA-Hsp65 showed immunostimulatory properties against infectious diseases, autoimmune diseases, cancer and antifibrotic properties in an egg-induced granuloma model. Methods: C57BL/6 mice received 4 doses of DNA-Sm14 (100 mu g/dose) and DNA-Hsp65 (100 mu g/dose), simultaneously administrated, or DNA-Sm14 alone, once a week, during four weeks. Three groups were included: 1-Control (no immunization); 2 DNA Sm14;3 DNA Sm14/DNA Hsp65. Two weeks following last immunization, animals were challenged subcutaneously with 30 cercariae. Fifteen, 48 and 69 days after infection splenocytes were collected to evaluate the number of CD8+ memory T cells (CD44(high)CD62(low)) using flow cytometry. Forty-eight days after challenge adult worms were collected by portal veins perfusion and intestines were collected to analyze the intestinal egg viability. Histological, immunohistochemical and soluble quantification of collagen and alpha-SMA accumulation were performed on the liver. Results: In the current work, we tested a new vaccination strategy using DNA-Sm14 with DNA-Hsp65 to potentiate the protection against schistosomiasis. Combined vaccination increased the number of CD8+ memory T cells and decreased egg viability on the intestinal wall of infected mice. In addition, simultaneous vaccination with DNA-Sm14/DNA-Hsp65 reduced collagen and alpha-SMA accumulation during the chronic phase of granuloma formation. Conclusion: Simultaneous vaccination with DNA- Sm14/ DNA- Hsp65 showed an immunostimulatory potential and antifibrotic property that is associated with the reduction of tissue damage on Schistosoma mansoni experimental infection. (AU)

Grantee:Milena Sobral Espíndola
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 09/07169-5 - Lipid mediators as regulators of immune response
Grantee:Lúcia Helena Faccioli
Support Opportunities: Research Projects - Thematic Grants