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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential modulation of sympathetic and respiratory activities by cholinergic mechanisms in the nucleus of the solitary tract in rats

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Author(s):
Furuya, Werner I. [1] ; Bassi, Mirian [1] ; Menani, Jose V. [1] ; Colombari, Eduardo [1] ; Zoccal, Daniel B. [1] ; Colombari, Debora S. A. [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Experimental Physiology; v. 99, n. 5, p. 743-758, MAY 1 2014.
Web of Science Citations: 8
Abstract

New Findings: What is the central question of this study? Is sympathorespiratory activity affected in a different manner by cholinergic mechanisms in the intermediate (iNTS) and commissural nucleus of the solitary tract (cNTS) and are cholinergic mechanisms involved in baro- and chemoreflexes? What is the main finding and its importance? Acetylcholine (ACh) injected into the iNTS promotes sympatho-inhibition and reduces the phrenic frequency, whereas ACh injected into the cNTS increases phrenic frequency and affects sympathetic-respiratory coupling, without changing the sympathetic activity. These responses are abolished by mecamylamine (nicotinic antagonist) in the NTS. Mecamylamine in the cNTS also reduces peripheral chemoreflex-induced tachypnoea. The contribution of cholinergic mechanisms of the nucleus of the solitary tract (NTS) to cardiorespiratory control is not completely clear. In the present study, we investigated the involvement of the cholinergic mechanisms in the intermediate NTS (iNTS) and commissural NTS (cNTS) on the control of sympathetic (SNA) and phrenic nerve activity (PNA). Decorticated, arterially perfused in situ preparations of male juvenile rats (60-100g) were used. Acetylcholine (10mm, 60nl) injected into the iNTS reduced SNA (-54 +/- 4%, versus vehicle -5 +/- 3%; P<0.001) and PNA (-30 +/- 4%, versus vehicle -5 +/- 6%; P<0.001), whereas injections of ACh into the cNTS increased PNA (30 +/- 6%, versus vehicle 5 +/- 3%; P<0.001), without changing SNA. Pretreatment with mecamylamine (nicotinic antagonist; 5mm) abolished all the effects of ACh injected into the iNTS or the cNTS, whereas atropine (muscarinic antagonist; 5mm) reduced only the effects of ACh injected into the cNTS. Mecamylamine injected into the cNTS also reduced the tachypnoea in response to peripheral chemoreflex activation. The baroreflex was unaltered by injections of atropine or mecamylamine into the NTS. The results suggest that ACh and mainly nicotinic receptors in the NTS are involved in the modulation of SNA and PNA, with distinct functions between the iNTS and the cNTS. An involvement of the nicotinic receptors in the cNTS in the tachypnoea in response to peripheral chemoreflex activation is also suggested. (AU)

FAPESP's process: 10/17218-0 - Cholinergic cardiorespiratory function in the NTS
Grantee:Werner Issao Furuya
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/54888-7 - Neural mechanisms involved on chemoreception
Grantee:Eduardo Colombari
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/20040-1 - Cardiorespiratory cholinergic function in the nucleus of the solitary tract
Grantee:Débora Simões de Almeida Colombari
Support type: Regular Research Grants