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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Vitamin B Deprivation during Pregnancy and Lactation on Homocysteine Metabolism and Related Metabolites in Brain and Plasma of Mice Offspring

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da Silva, Vanessa Cavalcante [1, 2] ; Fernandes, Leandro [2] ; Haseyama, Eduardo Jun [2] ; Dias Abdo Agamme, Ana Luiza [2] ; Guerra Shinohara, Elvira Maria [3] ; Cartaxo Muniz, Maria Tereza [4] ; D'Almeida, Vania [2, 1]
Total Authors: 7
[1] Univ Fed Sao Paulo, Dept Pediat, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo - Brazil
[4] Univ Pernambuco, Pediat Hematol & Oncol Ctr, Inst Biol Sci, Recife, PE - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 9, n. 4 APR 2 2014.
Web of Science Citations: 5

Epidemiological and experimental studies indicate that the altered fetal and neonatal environment influences physiological functions and may increase the risk of developing chronic diseases in adulthood. Because homocysteine (Hcy) metabolic imbalance is considered a risk factor for neurodegenerative diseases, we investigated whether maternal Vitamin B deficiency during early development alters the offspring's methionine-homocysteine metabolism in their brain. To this end, the dams were submitted to experimental diet one month before and during pregnancy or pregnancy/lactation. After birth, the offspring were organized into the following groups: control (CT), deficient diet during pregnancy and lactation (DPL) and deficient diet during pregnancy (DP). The mice were euthanized at various stages of development. Hcy, cysteine, glutathione (GSH), S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), folate and cobalamin concentrations were measured in the plasma and/or brain. At postnatal day (PND) 0, total brain of female and male offspring exhibited decreased SAM/SAH ratios. Moreover, at PND 28, we observed decreased GSH/GSSG ratios in both females and males in the DPL group. Exposure to a Vitamin B-deficient diet during the ontogenic plasticity period had a negative impact on plasma folate and brain cortex SAM concentrations in aged DPL males. We also observed decreased plasma GSH concentrations in both DP and DPL males (PND 210). Additionally, this manipulation seemed to affect the female and male offspring differently. The decreased plasma GSH concentration may reflect redox changes in tissues and the decreased brain cortex SAM may be involved in changes of gene expression, which could contribute to neurodegenerative diseases over the long term. (AU)

FAPESP's process: 10/00075-2 - Maternal hyperhomocysteinemia and epigenetic changes in fetal programming of genes involved in Alzheimer's Disease pathogenesis
Grantee:Vânia D'Almeida
Support type: Regular Research Grants