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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of methicillin-resistant Staphylococcus aureus resistant to tigecycline and daptomycin isolated in a hospital in Brazil

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Dabul, A. N. G. [1] ; Camargo, I. L. B. C. [1]
Total Authors: 2
[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: EPIDEMIOLOGY AND INFECTION; v. 142, n. 3, p. 479-483, MAR 2014.
Web of Science Citations: 12

We report the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) with resistance to tigecycline and to daptomycin isolated from intensive-care-unit patients in Brazil over a 6-month period. Thirty-six isolates (25 from infection sites, 11 from nasal sites) recovered from 23 patients who presented with MRSA infection during this period were characterized by pulsed-field gel electrophoresis, multilocus sequence typing, staphylococcal cassette chromosome mec (SCCmec) typing, and antimicrobial susceptibility profiling. Ten isolates from six patients and two isolates from different patients were resistant to tigecycline and daptomycin, respectively. Eight pulsotypes were identified and one, type A, accounted for 21 isolates from 12 patients; type A isolates were SCCmecII as were a further nine isolates of other pulsotypes. All but four of the total isolates were sequence type (ST) 5 or ST105 and classified as clonal complex (CC) 5; the historically prevalent lineage in Brazil, ST239-SCCmecIII, was identified in only three patients. Tigecycline-resistant strains were all ST105-SCCmecII and two patients were nasally colonized by strains of the same pulsotype found in infection sites. Two ST5-SCCmecII were daptomycin resistant after 48h incubation. The origin and mechanism of these resistant strains remains unknown and further studies are warranted to determine whether such clones are becoming endemic in Brazilian hospitals and to assess their impact on infection control practice. (AU)

FAPESP's process: 10/11890-9 - Molecular epidemiologic study of Methicillin-Resistant Staphylococcus aureus (MRSA) isolated in Brazil and study of the response regulator protein GraR
Grantee:Andrei Nicoli Gebieluca Dabul Dias de Sousa
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 10/02619-0 - Molecular epidemiological study of Brazilian strains of Methicillin-resistant Staphylococcus aureus (MRSA) isolated in Brazil and study of the response regulator protein GraR
Grantee:Ilana Lopes Baratella da Cunha Camargo
Support type: Research Grants - Young Investigators Grants