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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analytical methods for copper, zinc and iron quantification in mammalian cells

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Cerchiaro, Giselle [1] ; Manieri, Tania Maria [1] ; Bertuchi, Fernanda Rodrigues [1]
Total Authors: 3
[1] Fed Univ ABC UFABC, Ctr Nat Sci & Humanities, Santo Andre, SP - Brazil
Total Affiliations: 1
Document type: Review article
Source: METALLOMICS; v. 5, n. 10, p. 1336-1345, 2013.
Web of Science Citations: 34

Highly complex analytical methods with different accuracies of measurement, reproducibilities and ease of analyses are currently being used to quantify metals in cellular media and tissue samples. In this review, the analytical methods commonly used for iron, copper and zinc quantification in mammalian cells are presented and discussed. Herein, we present a literature survey of the most commonly found concentrations of these metals in various mammalian cells in culture and tissues. The aim of this review is to help researchers in metallomic-related areas identify the method that best suits their needs for the accurate quantification of these metals in cells. This accuracy goes beyond simple knowledge of the limit of detection of each technique and needs to be evaluated through comparisons with similar previous studies. (AU)

FAPESP's process: 12/01397-9 - Effects of metalloenzyme superoxide dismutase expression in the generation of reactive oxygen species in cultured mammalian cells and in motor neurons
Grantee:Giselle Cerchiaro
Support type: Regular Research Grants