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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Kinetic studies of recombinant rabies virus glycoprotein (RVGP) cDNA transcription and mRNA translation in Drosophila melanogaster S2 cell populations

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Astray, R. M. [1] ; Jorge, S. A. C. [1] ; Lemos, M. A. N. [1] ; Yokomizo, A. Y. [1] ; Boldorini, V. L. L. [1] ; Puglia, A. L. P. [1] ; Ribeiro, O. G. [2] ; Pereira, C. A. [1]
Total Authors: 8
[1] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Lab Imunogenet, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Cytotechnology; v. 65, n. 5, p. 829-838, OCT 2013.
Web of Science Citations: 4

Recombinant rabies virus glycoprotein (RVGP) was expressed in cell membranes of stably transfected Drosophila S2 cells using constitutive and inducible promoters. Although with quantitative differences of RVGP expression in both systems, the cDNA transcription, as evaluated by relative RVGP mRNA levels measured by qRT-PCR, sustained the amount of RVGP producing cells and the RVGP volumetric (I (RVGP)) productivity. At the transition to the stationary cell growth phase, once the cell culture slowed down its rate of multiplication, an accumulation of RVGP mRNA and RVGP was clearly observed in both cell populations. Nevertheless, cell cultures performed under sub-optimal temperatures indicated that an envisaged increase in the RVGP production is not only dependent on cell growth rate, but essentially on optimal cell metabolic state. (AU)

FAPESP's process: 11/08331-0 - Study of the quantitative dinamics of heterologous gene transcription in transfected animals cells for bioprocess optimization
Grantee:Carlos Augusto Pereira
Support Opportunities: Regular Research Grants