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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of Genotoxicity and Antigenotoxicity of Artepillin C in V79 Cells by the Comet and Micronucleus Assays

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de Oliveira, Pollyanna Francielli [1] ; de Souza Lima, Ildercilio Mota [1] ; Bentes Monteiro Neto, Moacir de Azevedo [1] ; Bastos, Jairo Kenupp [2] ; da Silva Filho, Ademar Alves [3] ; Tavares, Denise Crispim [1]
Total Authors: 6
[1] Univ Franca, BR-14404611 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil
[3] Univ Fed Juiz de Fora, Dept Ciencias Farmaceut, Fac Farm, Juiz De Fora, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL; v. 65, n. 7, p. 1098-1103, OCT 1 2013.
Web of Science Citations: 3

Artepillin C (3,5-diprenyl-p-coumaric acid) is one of the major phenolic compounds found in Brazilian green propolis, as well as in its botanical source, Baccharis dracunculifolia DC (Asteraceae). The present study evaluated the possible genotoxic and protective activities of artepillin C, in vitro, using methyl methanesulfonate (MMS) as a positive control, by comet and micronucleus assays. The cultures of Chinese hamster lung fibroblasts (V79 cells) were treated with different concentrations of artepillin C (2.5, 5.0, 10.0, and 20 M). In antigenotoxicity assessment, the 3 concentrations of artepillin C (2.5, 5.0, and 10.0M) were associated with MMS (200M-comet assay and 400M-micronucleus assay). A statistically significant increase in the DNA damage and micronucleus frequencies was observed in the culture treated with the highest concentration of the artepillin C in comparison to the control group. All concentrations of artepillin C showed protective activity in relation to MMS-induced genotoxicity, which may be due to its antioxidant properties. (AU)

FAPESP's process: 09/05944-1 - In vivo evaluation of mutagenic/genotoxic and antimutagenic/antigenotoxic potential of artepillin C
Grantee:Ildercílio Mota de Souza Lima
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 07/07211-6 - Study of mutagenic and/or antimutagenic activity of Baccharis dracunculifolia and Artepillin C in mammalian cells
Grantee:Denise Crispim Tavares Barbosa
Support Opportunities: Regular Research Grants
FAPESP's process: 09/12019-2 - In vivo and in vitro evaluation of mutagenic/genotoxic and antimutagenic/antigenotoxic potential of baccharin, an important chemical compound of Baccharis dracunculifolia
Grantee:Pollyanna Francielli de Oliveira
Support Opportunities: Scholarships in Brazil - Master