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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Stress tolerance and growth physiology of yeast strains from the Brazilian fuel ethanol industry

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Author(s):
Della-Bianca, B. E. [1] ; Gombert, A. K. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Dept Chem Engn, BR-05424970 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 104, n. 6, p. 1083-1095, DEC 2013.
Web of Science Citations: 15
Abstract

Improved biofuels production requires a better understanding of industrial microorganisms. Some wild Saccharomyces cerevisiae strains, isolated from the fuel ethanol industry in Brazil, present exceptional fermentation performance, persistence and prevalence in the harsh industrial environment. Nevertheless, their physiology has not yet been systematically investigated. Here we present a first systematic evaluation of the widely used industrial strains PE-2, CAT-1, BG-1 and JP1, in terms of their tolerance towards process-related stressors. We also analyzed their growth physiology under heat stress. These strains were evaluated in parallel to laboratory and baker's strains. Whereas the industrial strains performed in general better than the laboratory strains under ethanol or acetic acid stresses and on industrial media, high sugar stress was tolerated equally by all strains. Heat and low pH stresses clearly distinguished fuel ethanol strains from the others, indicating that these conditions might be the ones that mostly exert selective pressure on cells in the industrial environment. During shake-flask cultivations using a synthetic medium at 37 A degrees C, industrial strains presented higher ethanol yields on glucose than the laboratory strains, indicating that they could have been selected for this trait-a response to energy-demanding fermentation conditions. These results might be useful to guide future improvements of large-scale fuel ethanol production via engineering of stress tolerance traits in other strains, and eventually also for promoting the use of these fuel ethanol strains in different industrial bioprocesses. (AU)

FAPESP's process: 07/59776-7 - Yeast improvement by metabolic and evolutionary engineering
Grantee:Andreas Karoly Gombert
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 10/07187-0 - Tolerance of Saccharomyces cerevisiae towards stressors of the bioethanol industry
Grantee:Bianca Eli Della Bianca
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)