Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface

Full text
de Assis, Adriano Freitas ; Beloti, Marcio Mateus ; Crippa, Grasiele Edilaine ; de Oliveira, Paulo Tambasco ; Morra, Marco [1] ; Rosa, Adalberto Luiz [2]
Total Authors: 6
[1] Nobil Bioric Srl, Villafranca Dasti - Italy
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Cell Culture Lab, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Clinical Oral Implants Research; v. 20, n. 3, p. 240-246, Mar. 2009.
Field of knowledge: Health Sciences - Dentistry
Web of Science Citations: 15

The aim of this study was to evaluate the development of the osteoblastic phenotype in human alveolar bone-derived cells grown on collagen type I-coated titanium (Ti) surface (Col-Ti) obtained by plasma deposition acrylic acid grafting compared with machined Ti (M-Ti). Osteoblastic cells were cultured until subconfluence and subcultured on Col-Ti and M-Ti for periods of up to 21 days. Cultures grown on Col-Ti and M-Ti exhibited similar cell morphology. Cell adhesion, total protein content, and alkaline phosphatase (ALP) activity were not affected by Ti surface modification in all evaluated periods. Growth analyses indicated that there were significantly more cells in cultures grown on Col-Ti at day 3. Runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteoprotegerin (OPG) mRNA expression of cells subcultured on Col-Ti was higher, whereas collagen type I (COL) was lower compared with M-Ti. Ti surface modification neither affected the osteocalcin (OC), ALP and receptor activator of NF-kB ligand (RANKL)mRNA expression nor the calciumcontent extracted from mineralized matrix. These results demonstrated that Col-Ti favours cell growth during the proliferative phase (day 3) and osteoblastic differentiation, as demonstrated by changes in mRNA expression profile during the matrix mineralization phase (day 14), suggesting that this Ti surface modification may affect the processes of bone healing and remodelling. (AU)

FAPESP's process: 03/09767-0 - Ostogenesis on titanium: in vitro evaluation of the effects of different stimulatory methods
Grantee:Adalberto Luiz Rosa
Support Opportunities: Research Projects - Thematic Grants