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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development and Validation of Methods for the Extraction of Phenolic Acids from Plasma, Urine, and Liver and Analysis by UPLC-MS

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Author(s):
de Oliveira, Daniela M. [1] ; Pinto, Carolina B. [1] ; Sampaio, Geni R. [1] ; Yonekura, Lina [1] ; Catharino, Rodrigo R. [2] ; Bastos, Deborah H. M. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Sch Med Sci, INNOVARE Biomarkers Lab, BR-13083887 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Agricultural and Food Chemistry; v. 61, n. 25, p. 6113-6121, JUN 26 2013.
Web of Science Citations: 9
Abstract

This study developed and validated a method for the extraction and determination of 11 phenolic acids in rat plasma, urine, and liver by ultraperformance liquid, chromatography-mass spectrometry (UPLC-MS). A system suitability test (instrumental linearity, area, and retention time precision) was performed and recovery, intraday and between-day precisions, detection limits (LOD), and quantification limits (LOQ) were determined for all compounds in each biological matrix. Recoveries varied between 88 and 117% in plasma, between 87 and 102% in urine, and between 38 and 100% in liver. Precision was higher than 13.7% intraday and 14.0% interday in all matrices, at three concentration levels. To demonstrate the applicability, the method was used to estimate the concentrations of phenolic acids in samples from animals that received 5-caffeoylquinic acid (5-CQA) by gavage. The excellent validation results and the applicability of the method to real samples confirmed the suitability for studies on absorption, bioavailability, and pharmacokinetics of phenolic acids derived from foods rich in phenolic compounds. (AU)

FAPESP's process: 08/10308-4 - Bioaccessibility, bioavailability and antioxidant activity of phenolic compounds from Guaraná (Paullinia cupana) assessed in vitro and in vivo
Grantee:Lina Yonekura
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 09/01275-8 - In vitro and in vivo study of yerba mate (Ilex paraguariensis) phenolic compounds bioavailability.
Grantee:Daniela Moura de Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/14853-0 - Evaluation of yerba maté (Ilex paruaguariensis) phenolic acids bioavailability
Grantee:Deborah Helena Markowicz Bastos
Support Opportunities: Regular Research Grants