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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples

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Author(s):
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LRA Vaz-de-Lima [1] ; MCO Souza [2] ; TK Matsumoto [3] ; MA Hong [4] ; MM Salgado [5] ; ML Barbosa [6] ; NS Sato [7] ; HI Requejo [8] ; CAF Oliveira [9] ; R Pecchini [10] ; E Berezin [11] ; SD Passos [12] ; C Schvartsman [13] ; A Pasmanick [14] ; EL Durigon ; M Ueda [16]
Total Authors: 16
Affiliation:
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[1] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[2] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[3] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[4] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[5] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[6] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[7] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[8] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[9] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
[10] Irmandade Santa Casa de Misericórdia de São Paulo - Brasil
[11] Irmandade Santa Casa de Misericórdia de São Paulo - Brasil
[12] Faculdade de Medicina de Jundiaí. Hospital Universitário - Brasil
[13] Faculdade de Medicina. Instituto da Criança
[14] Faculdade de Medicina. Instituto da Criança
[16] Instituto Adolfo Lutz. Rede Diversidade Genômica de Vírus - Brasil
Total Affiliations: 16
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 103, n. 5, p. 463-467, 2008-08-00.
Abstract

Comparison of the use of indirect immunofluorescence assay (IFA), immunochromatography assay (ICA-BD) and reverse transcription-polymerase chain reaction (RT-PCR) for detecting human respiratory syncytial virus (HRSV) in 306 nasopharyngeal aspirates samples (NPA) was performed in order to assess their analytical performance. By comparing the results obtained using ICA-BD with those using IFA, we found relative indices of 85.0% for sensitivity and 91.2% for specificity, and the positive (PPV) and negative (NPV) predictive values were 85.0% and 91.2%, respectively. The relative indices for sensitivity and specificity as well as the PPV and NPV for RT-PCR were 98.0%, 89.0%, 84.0% and 99.0%, respectively, when compared to the results of IFA. In addition, comparison of the results of ICA-BD and those of RT-PCR yielded relative indices of 79.5% for sensitivity and 95.4% for specificity, as well as PPV and NPV of 92.9% and 86.0%, respectively. Although RT-PCR has shown the best performance, the substantial agreement between the ICA-BD and IFA results suggests that ICA-BD, also in addition to being a rapid and facile assay, could be suitable as an alternative diagnostic screening for HRSV infection in children. (AU)

FAPESP's process: 02/08462-9 - Viral Genetic Diversity Network (VGDN)
Grantee:Mirthes Ueda
Support type: Regular Research Grants