Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High-fat diet blunts activation of the nuclear factor-kappa B signaling pathway in lipopolysaccharide-stimulated peritoneal macrophages of Wistar rats

Full text
Borges, Maria C. [1] ; Vinolo, Marco A. R. [2] ; Crisma, Amanda R. [2] ; Fock, Ricardo A. [3] ; Borelli, Primavera [3] ; Tirapegui, Julio [4] ; Curi, Rui [2] ; Rogero, Marcelo M. [1]
Total Authors: 8
[1] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: NUTRITION; v. 29, n. 2, p. 443-449, FEB 2013.
Web of Science Citations: 11

Objective: The present study was designed to investigate the effect of a high-fat diet (HFD) on the inflammatory response of peritoneal macrophages. Methods: Male Wistar rats were fed a control diet (n = 12) or an HFD (n = 12) for 12 wk. After euthanasia, peritoneal macrophages were collected and stimulated (or not) with lipopolysaccharide (LPS). Results from the assays using peritoneal macrophages were analyzed with one-way analysis of variance or an equivalent non-parametric test. The level of significance adopted was 0.05. Results: Consumption of the HFD was associated with significant increases in weight gain and fat depots (P < 0.05). Despite having no influence in systemic markers of inflammation, such as interleukin (IL)-6, tumor necrosis factor-a, and plasminogen activator inhibitor-1, the HFD intake significantly decreased insulin sensitivity, as evaluated by the homeostasis model assessment index (P < 0.05). A decreased production of IL-1 beta, IL-6, IL-10, and nitric oxide in response to the LPS stimulation was observed in peritoneal macrophages from the HFD group (P < 0.05). Also, in HFD-fed animals, LPS incubation did not increase IL-1 beta and IL-6 mRNA expression (P < 0.05). These effects were associated with an attenuation of I kappa B inhibitor kinase-beta phosphorylation and nuclear factor-kappa B activation in response to LPS and with a failure to decrease I kappa B inhibitor-alpha expression (P < 0.05). Conclusion: Chronic consumption of an HFD decreased the LPS-induced inflammatory response of peritoneal macrophages, which was associated with a downregulation of the nuclear factor-kappa B signaling pathway. (C) 2013 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 09/02371-0 - The effect of yerba mate (Ilex paraguariensis) aqueous extract ingestion on nuclear factor kappa B signaling pathway in peritoneal macrophages of rats fed a high-fat diet
Grantee:Maria Carolina Borges
Support Opportunities: Scholarships in Brazil - Master