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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)


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Zanoni, Fernando Luiz [1] ; Benabou, Simon [1] ; Greco, Karin Vicente [1] ; Ramos Moreno, Ana Carolina [2] ; Miranda Costa Cruz, Jose Walber [1] ; Filgueira, Fernando Paranaiba [3] ; Martinez, Marina Baquerizo [2] ; Poli de Figueiredo, Luiz Francisco [1] ; Rocha e Silva, Mauricio [1] ; Sannomiya, Paulina [1]
Total Authors: 10
[1] Univ Sao Paulo, Sch Med, LIM 11, Heart Inst InCor, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Clinics; v. 64, n. 9, p. 911-919, 2009.
Web of Science Citations: 23

PRUPOSE: Bacterial translocation has been shown to occur in critically ill patients after extensive trauma, shock, sepsis, or thermal injury. The present study investigates mesenteric microcirculatory dysfunctions, the bacterial translocation phenomenon, and hemodynamic/metabolic disturbances in a rat model of intestinal obstruction and ischemia. METHODS: Anesthetized (pentobarbital 50 mg/kg, i.p.) male Wistar rats (250-350 g) were submitted to intestinal obstruction or laparotomy without intestinal obstruction (Sham) and were evaluated 24 hours later. Bacterial translocation was assessed by bacterial culture of the mesenteric lymph nodes (MLN), liver, spleen, and blood. Leukocyte-endothelial interactions in the mesenteric microcirculation were assessed by intravital microscopy, and P-selectin and intercellular adhesion molecule (ICAM)-1 expressions were quantified by immunohistochemistry. Hematocrit, blood gases, lactate, glucose, white blood cells, serum urea, creatinine, bilirubin, and hepatic enzymes were measured. RESULTS: About 86% of intestinal obstruction rats presented positive cultures for E. coli in samples of the mesenteric lymph nodes, liver, and spleen, and 57% had positive hemocultures. In comparison to the Sham rats, intestinal obstruction induced neutrophilia and increased the number of rolling (similar to 2-fold), adherent (similar to 5-fold), and migrated leukocytes (similar to 11-fold); this increase was accompanied by an increased expression of P-selectin (similar to 2-fold) and intercellular adhesion molecule-1 (similar to 2-fold) in the mesenteric microcirculation. Intestinal obstruction rats exhibited decreased PaCO2, alkalosis, hyperlactatemia, and hyperglycemia, and increased blood potassium, hepatic enzyme activity, serum urea, creatinine, and bilirubin. A high mortality rate was observed after intestinal obstruction (83% at 72 h vs. 0% in Sham rats). CONCLUSION: Intestinal obstruction and ischemia in rats is a relevant model for the in vivo study of mesenteric microcirculatory dysfunction and the occurrence of bacterial translocation. This model parallels the events implicated in multiple organ dysfunction (MOD) and death. (AU)

FAPESP's process: 04/15964-6 - Mechanisms of hypertonic saline solution associated to pentoxifylline and ethy-pyruvate in the reduction of multiple organ dysfunctions after trauma, sepsis and ischemia-reperfusion
Grantee:Maurício Rocha e Silva
Support Opportunities: Research Projects - Thematic Grants