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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of Human Bocavirus mRNA in Respiratory Secretions Correlates with High Viral Load and Concurrent Diarrhea

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Proenca-Modena, Jose Luiz [1, 2] ; Gagliardi, Talita Bianca [1, 2] ; de Paula, Flavia Escremim [1, 2] ; Iwamoto, Marisa Akiko [2] ; Criado, Miria Ferreira [1, 2] ; Camara, Ataide A. [3] ; Acrani, Gustavo Olszanski [1, 2] ; Leite Cintra, Otavio Augusto [4] ; Cervi, Maria Celia [4] ; de Paula Arruda, Luisa Karla [5] ; Arruda, Eurico [1, 2]
Total Authors: 11
[1] Univ Sao Paulo, Sch Med, Dept Cell Biol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Virol Res Ctr, Sao Paulo - Brazil
[3] Santa Lydia Hosp, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Pediat, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Internal Med, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PLoS One; v. 6, n. 6 JUN 20 2011.
Web of Science Citations: 27

Human bocavirus (HBoV) is a parvovirus recently identified in association with acute respiratory infections (ARI). Despite its worldwide occurrence, little is known on the pathogenesis of HBoV infections. In addition, few systematic studies of HBoV in ARI have been conducted in Latin America. Therefore, in order to test whether active viral replication of human bocavirus is associated with respiratory diseases and to understand the clinical impact of this virus in patients with these diseases, we performed a 3-year retrospective hospital-based study of HBoV in outpatients and inpatients with symptoms of Acute Respiratory Infections (ARI) in Brazil. Nasopharyngeal aspirates (NPAs) from 1015 patients with respiratory symptoms were tested for HBoV DNA by PCR. All samples positive for HBoV were tested by PCR for all other respiratory viruses, had HBoV viral loads determined by quantitative real time PCR and, when possible, were tested by RT-PCR for HBoV VP1 mRNA, as evidence of active viral replication. HBoV was detected in 4.8% of patients, with annual rates of 10.0%, 3.0% and 3.0% in 2005, 2006 and 2007, respectively. The range of respiratory symptoms was similar between HBoV-positive and HBoV-negative ARI patients. However, a higher rate of diarrhea was observed in HBoV-positive patients. High HBoV viral loads (> 10(8) copies/mL) and diarrhea were significantly more frequent in patients with exclusive infection by HBoV and in patients with detection of HBoV VP1 mRNA than in patients with viral co-infection, detected in 72.9% of patients with HBoV. In summary, our data demonstrated that active HBoV replication was detected in a small percentage of patients with ARI and was correlated with concurrent diarrhea and lack of other viral co-infections. (AU)

FAPESP's process: 08/50617-6 - Studies on emerging viruses including arbovirus, robovirus, respiratory viruses and congenital transmission, at the Centro de Pesquisa em Virologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo
Grantee:Luiz Tadeu Moraes Figueiredo
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 09/51818-8 - The profile of viral infections in chronic diseases of the upper respiratory tract: adenoamigdaliana hypertrophy and secretory otitis media, rhinosinusitis
Grantee:Wilma Terezinha Anselmo Lima
Support Opportunities: Research Projects - Thematic Grants