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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

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Stafuzza, N. B. [1] ; Abbey, C. A. [2] ; Gill, C. A. [2] ; Womack, J. E. [3] ; Amaral, M. E. J. [1]
Total Authors: 5
[1] Univ Estadual Sao Paulo Julio de Mesquita Filho, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil
[2] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 - USA
[3] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX - USA
Total Affiliations: 3
Document type: Journal article
Source: Genetics and Molecular Research; v. 11, n. 3, p. 3013-3019, 2012.
Web of Science Citations: 9

River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 x coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements. (AU)

FAPESP's process: 11/02478-0 - Molecular analysis of the buffalo MHC region using next generation genomic tools
Grantee:Nedenia Bonvino Stafuzza
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 08/10725-4 - Genomic Studies in buffalo: application of recent buffalo genomic maps to characterize economically and biologically important genes using genomic library tools.
Grantee:Maria Elisabete Jorge Amaral
Support Opportunities: Regular Research Grants