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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The expression of antioxidant enzymes in the gingivae of type 2 diabetics with chronic periodontitis

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Duarte, Poliana M. [1] ; Napimoga, Marcelo H. [2] ; Fagnani, Ellen C. [3] ; Santos, Vanessa R. [1] ; Bastos, Marta F. [1] ; Ribeiro, Fernancla V. [1] ; Araujo, Vera C. [3] ; Demasi, Ana Paula D. [3]
Total Authors: 8
[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Sao Paulo - Brazil
[2] Sao Leopoldo Mandic Inst & Res Ctr, Lab Immunol & Mol Biol, BR-13045755 Campinas, SP - Brazil
[3] Sao Leopoldo Mandic Inst & Res Ctr, Pathol Lab, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 2, p. 161-168, FEB 2012.
Web of Science Citations: 8

Objectives: There is controversial evidence regarding the levels of antioxidant molecules in type 2 diabetes periodontitis patients. Thus, the aim of the present study was to evaluate the gene expression of antioxidant enzymes in the gingival tissue of poorly and well-controlled type 2 diabetic subjects with chronic periodontitis (CP). Design: Gingival biopsies were harvested from systemically and periodontally healthy subjects (n = 12), systemically healthy subjects with CP (n = 15), well-controlled (n = 8) and poorly controlled (n = 14) diabetic subjects with CP. The messenger RNA (mRNA) levels of peroxiredoxin (PRDX) 1 and 2, catalase (CAT), glutathione peroxidase (GPX1) and superoxide dismutase (SOD) 1 and 2 were measured by quantitative polymerase chain reaction (qPCR). Results: The results showed that PRDX1 and GPX1 were up-regulated by periodontitis (p < 0.05), independently of the glycaemic status, whilst PRDX2 and SOD2 genes were slightly influenced by periodontitis, but significantly induced when periodontitis was associated with DM, especially under a poor glycaemic control (p < 0.05). Moreover, CAT and SOD1 expressions were not significantly influenced by any of these inflammatory disorders (p > 0.05). Conclusion: In conclusion, both PRDX1 and GPX1 were overexpressed in CP whilst PRDX2 and SOD2 were up-regulated especially in the poorly controlled diabetic group with CP. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/04280-0 - Gene expression in sites with chronic periodontitis in diabetic individuals
Grantee:Poliana Mendes Duarte
Support Opportunities: Regular Research Grants
FAPESP's process: 09/52200-8 - Study of the function of peroxiredoxins I and IV in myeloma cells: evaluation of their potential as therapeutic targets
Grantee:Ana Paula Dias Demasi
Support Opportunities: Research Grants - Young Investigators Grants