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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Insights into the organization of dorsal spinal cord pathways from an evolutionarily conserved raldh2 intronic enhancer

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Castillo, Hozana A. [1, 2] ; Cravo, Roberta M. [1, 2] ; Azambuja, Ana P. [1, 2] ; Simoes-Costa, Marcos S. [1, 2] ; Sura-Trueba, Sylvia [1] ; Gonzalez, Jose [3] ; Slonimsky, Esfir [3] ; Almeida, Karla [4] ; Abreu, Jose G. [4] ; Afonso de Almeida, Marcio A. [1] ; Sobreira, Tiago P. [1] ; Pires de Oliveira, Saulo H. [1] ; Lopes de Oliveira, Paulo S. [1] ; Signore, Iskra A. [5] ; Colombo, Alicia [5] ; Concha, Miguel L. [5] ; Spengler, Tatjana S. [6] ; Bronner-Fraser, Marianne [6] ; Nobrega, Marcelo [7] ; Rosenthal, Nadia [3] ; Xavier-Neto, Jose [1]
Total Authors: 21
[1] InCor FMUSP, Lab Genet & Cardiol Mol, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, ICB, Dept Biol Celular & Desenvolvimento, BR-05508000 Sao Paulo - Brazil
[3] European Mol Biol Lab, Mouse Biol Programme, Monterotondo, RM - Italy
[4] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, BR-21941590 Rio De Janeiro - Brazil
[5] Univ Chile, Fac Med, Anat & Dev Biol Program ICBM, Lab Expt Ontogeny, Santiago 7 - Chile
[6] CALTECH, Div Biol, Pasadena, CA 91125 - USA
[7] Univ Chicago, Dept Human Genet, Chicago, IL 60637 - USA
Total Affiliations: 7
Document type: Journal article
Source: DEVELOPMENT; v. 137, n. 3, p. 507-518, FEB 1 2010.
Web of Science Citations: 15

Comparative studies of the tetrapod raldh2 (aldh1a2) gene, which encodes a retinoic acid (RA) synthesis enzyme, have led to the identification of a dorsal spinal cord enhancer. Enhancer activity is directed dorsally to the roof plate and dorsal-most (dl1) interneurons through predicted Tcf- and Cdx-homeodomain binding sites and is repressed ventrally via predicted Tgif homeobox and ventral Lim-homeodomain binding sites. Raldh2 and Math1/Cath1 expression in mouse and chicken highlights a novel, transient, endogenous Raldh2 expression domain in dl1 interneurons, which give rise to ascending circuits and intraspinal commissural interneurons, suggesting roles for RA in the ontogeny of spinocerebellar and intraspinal proprioceptive circuits. Consistent with expression of raldh2 in the dorsal interneurons of tetrapods, we also found that raldh2 is expressed in dorsal interneurons throughout the agnathan spinal cord, suggesting ancestral roles for RA signaling in the ontogenesis of intraspinal proprioception. (AU)