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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Central NO-cGMP pathway in thermoregulation and survival rate during polymicrobial sepsis

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Author(s):
Oliveira-Pelegrin, G. R. [1] ; Branco, L. G. S. [2] ; Rocha, M. J. A. [2]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040904 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Morfol Estomatol & Fisiol, Fac Odontol Ribeirao Preto, BR-14040904 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Canadian Journal of Physiology and Pharmacology; v. 88, n. 2, SI, p. 113-120, FEB 2010.
Web of Science Citations: 1
Abstract

Sepsis induces production of inflammatory mediators such as nitric oxide (NO) and causes physiological alterations, including changes in body temperature (T(b)). We evaluated the involvement of the central NO cGMP pathway in thermoregulation during sepsis induced by cecal ligation and puncture (CLP), and analyzed its effect on survival rate. Male Wistar rats with a T(b) probe inserted in their abdomen were intracerebroventricularly injected with 1 mu L N(G)-nitro-L-arginine methyl ester (L-NAME, 250 mu g), a nonselective NO synthase (NOS) inhibitor; or aminoguanidine (250 mu g), an inducible NOS inhibitor; or 1H-{[}1,2,4]oxadiazolo{[}4,3,-a]quinoxalin-1-one (ODQ, 0.25 mu g), a guanylate cyclase inhibitor. Thirty minutes after injection, sepsis was induced by cecal ligation and puncture (CLP), or the rats were sham operated. The animals were divided into 2 groups for determination of T(b) for 24 h and assessment of survival during 3 days. The drop in T(b) seen in the CLP group was attenuated by pretreatment with the NOS inhibitors (p < 0.05) and blocked with ODQ. CLP rats pretreated with either of the inhibitors showed higher survival rates than vehicle injected groups (p < 0.05), and were even higher in the ODQ pretreated group. Our results showed that the effect of NOS inhibition on the hypothermic response to CLP is consistent with the role of nitrergic pathways in thermoregulation. (AU)

FAPESP's process: 07/08532-0 - Neuroendocrine alterations during experimental sepsis induced by cecal ligation and puncture
Grantee:Maria José Alves da Rocha
Support type: Regular Research Grants