| Full text | |
| Author(s): |
Oliveira, Deise A. A. Pires
[1]
;
de Oliveira, Rodrigo Franco
[1]
;
Zangaro, Renato Amaro
[2]
;
Soares, Cristina Pacheco
[1]
Total Authors: 4
|
| Affiliation: | [1] UNIVAP, Inst Pesquisa & Desenvolvimento, Lab Dinam Compartimento Celular, BR-12244000 Sao Paulo - Brazil
[2] UNIVAP, Inst Pesquisa & Desenvolvimento, Lab Fluorescencia, BR-12244000 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Photomedicine and Laser Surgery; v. 26, n. 4, p. 401-404, AUG 2008. |
| Web of Science Citations: | 39 |
| Abstract | |
Objective: The purpose of the present study was to evaluate the effect of biomodulation on osteoblastic cells using a gallium-aluminium-arsenide diode laser. Background Data: Low-level laser therapy (LLLT) is a non-pharmacological therapeutic resource to which biological tissues respond well, producing such effects as the acceleration of bone formation and bone repair. Materials and Methods: Osteoblastic cell cultures (OFCOL II) were irradiated with a gallium-aluminium-arsenide diode laser (GaAlAs lambda = 830 nm; 50 mW; 3 J/cm(2); 600-mu m-diameter optical fiber) and divided into two groups: group 1 - irradiated cells, and group 2 - non-irradiated cells. Irradiation occurred at 24-h intervals for a total of 3 d. After each interval, the cells were marked with Mito Tracker Orange dye to assess the biostimulatory effect on mitochondrial activity and cell proliferation using an MTT assay. Results: Intense grouping of mitochondria in the perinuclear region was observed at 24 h and 48 h following irradiation. Changes from a filamentous to a granular appearance in mitochondrial morphology and mitochondria distributed throughout the cytoplasm were observed 72 h following proliferation. Such changes led to an in vitro proliferation process, as confirmed by the MTT assay. Conclusion: LLLT has shown itself capable of altering mitochondrial activity and the population of OFCOL II cells. (AU) | |