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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of Paracoccidioides brasiliensis COX9, COX12, and COX16 respiratory genes

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Bandeira, Simone C. B. [1] ; Nobrega, Marina P. [1]
Total Authors: 2
[1] Univ Sao Paulo, Inst Biociencias, Programa Microbiol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: MYCOLOGICAL RESEARCH; v. 112, n. 12, p. 1414-1420, DEC 2008.
Web of Science Citations: 2

Paracoccidioides brasiliensis is a thermo-dimorphic fungus that is the causative agent of paracoccidioidomyicosis (PCM), a human systemic granulomatous mycosis found in Latin America. Dimorphic transition from mycelium to yeast is required for establishing pathogenicity. Dimorphism is marked by changes in mitochondrial physiology, including modulation of respiration rate. In this work, we present the identification of three P. brasiliensis nuclear genes PbCOX9, PbCOX12, and PbCOX16 that code for structural sub-units and a putative assembly facilitator (PbCOX16) of the mitochondrial cytochrome c oxidase (COX), the terminal enzyme complex of the respiratory chain. We measured their expression pattern during the dimorphic transition from mycelium to yeast and back by real-time reverse transcription quantitative polymerase chain reaction (real-time RT-qPCR). Our results show that messages from these genes increase during the mycelium to yeast transition and decrease during the opposite conversion. This result supports active mitochondrial participation in the transition. Heterologous complementation of the corresponding Saccharomyces cerevisiae null mutant with the PbCOX9 gene was successfully obtained. (C) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 02/08711-9 - Molecular characterization of genes involved in the pathogeneticity/virulence in the pathogenetic fungus Paracoccidioides brasiliensis
Grantee:Gustavo Henrique Goldman
Support type: Research Projects - Thematic Grants