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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hepatotoxin microcystin-LR extraction optimization

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Silva-Stenico, Maria Estela [1] ; Cantusio Neto, Romeu [2] ; Alves, Ivanio R. [2] ; Moraes, Luiz Alberto B. [3] ; Shishido, Tania K. [1] ; Fiore, Marli Fatima [1]
Total Authors: 6
[1] Univ Sao Paulo, CENA, BR-13400970 Piracicaba, SP - Brazil
[2] Sociedade Abastecimento Agua & Saneamento SANASA, Cent Lab, BR-13045610 Campinas, SP - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 20, n. 3, p. 535-542, 2009.
Web of Science Citations: 20

Several cyanobacterial genera produce toxic secondary metabolites, the most well-known of which are the hepatotoxic microcystins (MCYSTs). Microcystin analyses in drinking water are a requirement of the Health Ministry (Regulation 518/2004) in Brazil, but this regulation does not establish which extraction and analytical method should be used; toxin quantification is usually carried out by ELISA (enzyme-linked immunosorbent assay) or HPLC (high performance liquid chromatography), the efficiency of which depends on the extraction method used. In this work a simple, fast and cheap method of extraction was developed for the isolation and identification of MCYSTs. For this, the strain Microcystis aeruginosa NPLJ-4, reported to be a MCYST-LR producer, was selected. Eight different treatments were tested to determine the best MCYST extraction. Samples were applied in LC-MS (liquid chromatography-mass spectrometry), ELISA and Q-TOF (quadrupole time-of-flight). The most efficient extraction was achieved by sonicating samples diluted in water. The proposed method permits rapid sample processing, and establishes an extraction method for both the analysis and identification of MCYST-LR and other variants. (AU)

FAPESP's process: 03/12529-4 - Nanotechnology: manufacture of nanostructures and supramolecular structures in liquid medium: observation of structures formed and investigation of processes of formation of nanostructures
Grantee:Omar Teschke
Support Opportunities: Research Projects - Thematic Grants