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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laser Biomodulation on L 929 Cell Culture

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Author(s):
Pires-Oliveira, Deise. A. A. [1] ; Oliveira, Rodrigo F. [1] ; Machado, Aline H. A. [2] ; Zangaro, Renato A. [1] ; Pacheco-Soares, Cristina [2]
Total Authors: 5
Affiliation:
[1] Univ Camilo Castelo Branca, Sao Paulo - Brazil
[2] UNIVAP, Sao Jose Dos Campos - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Photomedicine and Laser Surgery; v. 28, n. 2, p. 167-171, APR 2010.
Web of Science Citations: 7
Abstract

Objective: The aim of the present study was to analyze the effects of photobiomodulation using a 904-nm diode laser at two energy densities (6 J/cm(2) and 50 mJ/cm(2)) on L929 fibroblast cells. Background: Low-power laser irradiation (LPLI) is a non-pharmacological resource that induces important in vitro photobiomodulation on cell cultures and tissues. Methods: Irradiation was performed for three days at 24-h intervals. After each interval, the cells were stained with MitoTracker Orange (TM) and DioC6 dyes to assess the photobiomodulatory effects of irradiation on mitochondrial activity and changes in the endoplasmic reticulum. The MTT assay {[}3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide] was used to evaluate cell proliferation. Results and Conclusions: The fluorescence microscopy assessment of mitochondria and endoplasmic reticulum in cells irradiated with 6 J/cm(2) and 50 mJ/cm(2) demonstrated intense mitochondrial activity, which was confirmed by DioC6 staining. Reticular activity was observed stemming from increased protein synthesis. Photobiomodulation with 50 mJ/cm(2) was slightly higher than with 6 J/cm(2), as demonstrated by fluorescence microscopy results. Photobiomodulation was also time-dependent, with better results 72-h after irradiation. (AU)