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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of central NO-cGMP pathway in vasopressin and oxytocin gene expression during sepsis

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Author(s):
Oliveira-Pelegrin, Gabriela Ravanelli [1] ; Aguila, Fabio Alves [1] ; Basso, Paulo Jose [1] ; Alves Rocha, Maria Jose [1]
Total Authors: 4
Affiliation:
[1] USP, Fac Odontol Ribeirao Preto, Dept MEF, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Peptides; v. 31, n. 10, p. 1847-1852, OCT 2010.
Web of Science Citations: 19
Abstract

Sepsis induces massive production of inflammatory mediators, such as nitric oxide (NO), and causes neuroendocrine and cardiovascular alterations. This study investigates the involvement of the central NO-cGMP pathway in arginine vasopressin (AVP) and oxytocin (OXY) gene expression during sepsis induced by cecal ligation and puncture (CLP). Male Wistar rats received an i.c.v. injection of ODQ (0.25 mu g/mu L), a selective inhibitor of the heme site of soluble guanylate cyclase, or of 1% dymethilsulfoxide (DMSO), as vehicle. Thirty minutes after the injections, sepsis was induced by cecal ligation and puncture or the animals were sham operated. The ODQ pre-treatment did not alter the progressive NO increase observed after CLP. In the supraoptic nucleus (SON), this pretreatment increased the relative gene expression ratio of AVP and OXY in the initial phase of sepsis, but in the late phase, the gene expression of both hormones was reduced. In the paraventricular nucleus (PVN), soluble guanylate cyclase inhibition caused an even larger decrease in the relative gene expression ratio of AVP and OXY during sepsis. These results are indicative of a role of the NO-cGMP pathway in hormonal synthesis in the SON and PVN of the hypothalamus during polymicrobial sepsis. (C) 2010 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 07/08532-0 - Neuroendocrine alterations during experimental sepsis induced by cecal ligation and puncture
Grantee:Maria José Alves da Rocha
Support type: Regular Research Grants