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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunological and Microbiologic Changes during Caries Development in Young Children

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Parisotto, T. M. [1] ; King, W. F. [2] ; Duque, C. [1] ; Mattos-Graner, R. O. [1] ; Steiner-Oliveira, C. [1] ; Nobre-dos-Santos, M. [1] ; Smith, D. J. [2]
Total Authors: 7
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Piracicaba - Brazil
[2] Forsyth Inst, Boston, MA - USA
Total Affiliations: 2
Document type: Journal article
Source: Caries Research; v. 45, n. 4, p. 377-385, 2011.
Web of Science Citations: 23

We explored the association between caries development, colonization with caries-associated microflora, and immunity as children begin the transition to mixed dentition. Forty children received dental examinations at 3-4 years of age, repeated a year later. Children were grouped into caries-free (n = 23; CF) and caries-active (n = 17; CA >= 3 new lesions on follow-up). Salivary IgA and IgA antibody to Streptococcus mutans virulence epitopes were measured by Luminex assay. Mutans streptococci (MS), lactobacilli and total microorganisms were enumerated on selective media from plaque samples. There was no significant difference in baseline levels of MS or lactobacilli between CF and CA groups. However, both MS and lactobacilli levels were higher at follow-up in the CA group. Furthermore, children with detectable lactobacilli at baseline had significantly higher caries risk. Salivary IgA concentrations increased significantly in both groups during the study. Both CF and CA groups also displayed significant increases in salivary IgA antibody levels to glucosyltransferase, glucan-binding protein (Gbp) and antigen I/II salivary binding region. CF antibody levels to seven peptides associated with domains of biological importance increased at follow-up, in contrast to increases to only three peptides in CA saliva samples. Multivariate modeling showed that a lower baseline level of salivary IgA anti-GbpB was associated with higher caries risk. These data indicate that MS and lactobacilli are associated with caries in this population, that the secretory immune system is undergoing significant maturation during this period, and that the breadth of mucosal IgA response to epitopes of S. mutans virulence components may influence the degree to which these cariogenic microorganisms can cause disease. Copyright (C) 2011 S. Karger AG, Basel (AU)

FAPESP's process: 08/09510-3 - Relationship among biochemical composition of dental biofilm, immunological and environmental factors and caries incidence in preschool children with early childhood caries
Grantee:Thaís Parisotto Ulmer
Support type: Scholarships in Brazil - Doctorate