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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dipeptidyl peptidase IV in the hypothalamus and hippocampus of monosodium glutamate obese and food-deprived rats

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Alponti, Rafaela Fadoni [1, 2] ; Frezzatti, Rodrigo [1] ; Barone, Juliana Marton [1] ; Alegre, Valter de Sousa [1] ; Silveira, Paulo Flavio [1]
Total Authors: 5
[1] Inst Butantan, Pharmacol Lab, BR-05503900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Neurosci & Behav, BR-05508030 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: METABOLISM-CLINICAL AND EXPERIMENTAL; v. 60, n. 2, p. 234-242, FEB 2011.
Web of Science Citations: 14

Proline-specific dipeptidyl peptidases are emerging as a protease family with important roles in the regulation of signaling by peptide hormones related to energy balance. The treatment of neonatal rats with monosodium glutamate (MSG) is known to produce a selective damage on the arcuate nucleus with development of obesity. This study investigates the relationship among dipeptidyl peptidase IV (DPPIV) hydrolyzing activity, CD26 protein, fasting, and MSG model of obesity in 2 areas of the central nervous system. Dipeptidyl peptidase IV and CD26 were, respectively, evaluated by fluorometry, and enzyme-linked immunosorbent assay and reverse transcriptase polymerase chain reaction in soluble (SF) and membrane-bound (MF) fractions from the hypothalamus and hippocampus of MSG-treated and normal rats, submitted or not to food deprivation (FD). Dipeptidyl peptidase IV in both areas was distinguished kinetically as insensitive (DI) and sensitive (DS) to diprotin A. Compared with the controls, MSG and/or FD decreased the activity of DPPIV-DI in the SF and MF from the hypothalamus, as well as the activity of DPPIV-DS in the SF from the hypothalamus and in the MF from the hippocampus. Monosodium glutamate and/or FD increased the activity of DPPIV-DI in the MF from the hippocampus. The monoclonal protein expression of membrane CD26 by enzyme-linked immunosorbent assay decreased in the hypothalamus and increased in the hippocampus of MSG and/or FD relative to the controls. The existence of DPPIV-like activity with different sensitivities to diprotin A and the identity of insensitive with CD26 were demonstrated for the first time in the central nervous system. Data also demonstrated the involvement of DPPIV-DI/CD26 hydrolyzing activity in the energy balance probably through the regulation of neuropeptide Y and beta-endorphin levels in the hypothalamus and hippocampus. (C) 2011 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 05/04699-2 - Hypothalamic and hippocampal neutral aminopeptidase (APN/CD13), prolyl dipeptidyl aminopeptidase IV (DPPIV/CD26) and C-FOS in monosodium glutamate-treated and food-deprived rats
Grantee:Paulo Flávio Silveira
Support Opportunities: Regular Research Grants