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Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and-Gonadal Axes in Zebrafish

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Author(s):
Rodrigues, Maira S. ; Tovo-Neto, Aldo ; Rosa, Ivana F. ; Doretto, Lucas B. ; Fallah, Hamideh P. ; Habibi, Hamid R. ; Nobrega, Rafael H.
Total Authors: 7
Document type: Journal article
Source: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY; v. 10, p. 16-pg., 2022-05-12.
Abstract

In vertebrates, thyroid hormones are critical players in controlling different physiological processes such as development, growth, metabolism among others. There is evidence in mammals that thyroid hormones are also an important component of the hormonal system that controls reproduction, although studies in fish remain poorly investigated. Here, we tested this hypothesis by investigating the effects of methimazole-induced hypothyroidism on the testicular function in adult zebrafish. Treatment of fish with methimazole, in vivo, significantly altered zebrafish spermatogenesis by inhibiting cell differentiation and meiosis, as well as decreasing the relative number of spermatozoa. The observed impairment of spermatogenesis by methimazole was correlated with significant changes in transcript levels for several genes implicated in the control of reproduction. Using an in vitro approach, we also demonstrated that in addition to affecting the components of the brain-pituitary-peripheral axis, T3 (triiodothyronine) also exerts direct action on the testis. These results reinforce the hypothesis that thyroid hormones are an essential element of multifactorial control of reproduction and testicular function in zebrafish and possibly other vertebrate species. (AU)

FAPESP's process: 17/15793-7 - Effects of hypothyroidism on testicular function of Danio rerio and relation between thyroid hormones and follicle stimulating hormone (Fsh)
Grantee:Maira da Silva Rodrigues
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species
Grantee:Rafael Henrique Nóbrega
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/15319-6 - Cross-talk between gonadal gonadotropin-inhibitory hormone (GnIH) and thyroid hormones (THs) in the hypothalamic-pituitary-peripheral axis of zebrafish (Danio rerio)
Grantee:Maira da Silva Rodrigues
Support Opportunities: Scholarships abroad - Research Internship - Master's degree
FAPESP's process: 20/03569-8 - Testicular organoids and novel in vitro approaches to study spermatogonial stem cells and spermatogenesis in fish
Grantee:Rafael Henrique Nóbrega
Support Opportunities: Regular Research Grants