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Unveiling the Bacterial Sesquiterpenome of Streptomyces sp. CBMAI 2042 Discloses Cyclases with Versatile Performances

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Sachito, Douglas C. ; de Oliveira, Luciana G.
Total Authors: 2
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 33, n. 8, p. 9-pg., 2022-08-01.

Terpenes are the most abundant class of natural product that exist in nature. They possess a myriad of industrial applications including pharmaceutical, perfumery and flavors, bulk chemicals, and fuel. Intriguingly, until today, the vast majority of characterized terpenoids have been isolated from plants and fungi, and only in recent years bacteria were found to generate a representative reservoir of terpenoids molecules. Mining Streptomyces sp. CBMAI 2042 genome data has revealed the presence of five terpene cyclase genes. Chemical analysis of mycelium extract of this bacteria strain has unveiled at least 28 volatile terpenes molecules, where three encoding sesquiterpene cyclase (STC) genes are apparently responsible for their biosynthesis. The cyclic products obtained by incubation of these three purified recombinant STCs with farnesyl diphosphate (FPP) were analyzed by gas chromatography-mass spectrometry (GC-MS) and identified using the Van den Dool and Kratz equation. (AU)

FAPESP's process: 19/04855-7 - Exploration and expanding the terpenome of Streptomyces sp. CBMAI 2042 via genome mining
Grantee:Douglas Cunha Sachito
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/10564-5 - Assessing the beneficial aspects of endophytic actinobacteria: expanding the Brazilian microorganisms genome codified natural products
Grantee:Luciana Gonzaga de Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 14/12727-5 - Genome mining in Streptomyces
Grantee:Luciana Gonzaga de Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
Grantee:Arlene Gonçalves Corrêa
Support Opportunities: Research Grants - Research Centers in Engineering Program