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Screen-Printed Electrodes on Tyvek Substrate as Low-Cost Device to Applications in Alzheimer's Disease Detection

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Palley, Bianca Fortes ; Artur, Julio Cesar ; de Arruda, Milena Nakagawa ; de Souza, Gustavo Freitas ; Graves, David Alexandro ; de Carvalho Bovolato, Ana Livia ; Deffune, Elenice ; Schelp, Arthur Oscar ; Goncalves, Emerson Sarmento ; de Moraes, Marli Leite
Total Authors: 10
Document type: Journal article
Source: Journal of the Electrochemical Society; v. 169, n. 3, p. 7-pg., 2022-03-01.

Alzheimer' s disease (AD) is a progressive neurodegenerative disease characterized by impairment of cognitive functions and memory deterioration, which requires an early diagnosis for effective treatment. The use of immunosensors to detect Alzheimer's disease biomarkers stand out as a quick and cheap alternative for early detection of the disease. The development of Screen-Printed Electrodes (SPEs) meets a growing demand in the market for applications such as signal transducers in biosensor devices. In this work was development a method for fabrication of low cost SPE for application in the detection of Alzheimer' s disease through autoantibodies. It was produced microelectrodes on polyvinyl chloride (PVC), polyvinylidene fluoride (PVDF) and high-density polyethylene (HDPE) manufactured by DuPont Tyvek (R) substrates. SPEs produced on Tyvek substrates have shown promising results for low-cost, disposable and flexible sensors. The carbon paste showed excellent adhesion to Tyvek (R) substrate and the electrodes produced showed an electrochemical performance comparable to commercial electrodes, besides reproducible. The detection results showed that it is possible to detect anti-A beta 40 autoantibodies in real samples of serum and cerebrospinal fluid using the electrode modified with two bilayers of (PEI/(DDPG + A beta 40)) since the capacitance increased more in positive sample with the presence of autoantibody than in negative samples. (C) 2022 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited. (AU)

FAPESP's process: 17/17661-0 - Obtainment of reduced graphene oxide to sensor systems of aerospace industry
Grantee:Emerson Sarmento Gonçalves
Support Opportunities: Regular Research Grants
FAPESP's process: 19/27394-5 - High performance porous carbon accumulators for energy support for complex hubs powered by water desalination system
Grantee:Emerson Sarmento Gonçalves
Support Opportunities: Regular Research Grants
FAPESP's process: 21/04427-5 - Obtaining and characterizing nanostructured carbon electrodes for capacitive deionization processes
Grantee:David Alexandro Graves
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
FAPESP's process: 18/04648-9 - Immunosensor for the Alzheimer's Disease diagnosis in the preclinical stage by the detection of autoantibodies
Grantee:Marli Leite de Moraes
Support Opportunities: Regular Research Grants