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Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching

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Ortecho-Zuta, Uxua ; de Oliveira Duque, Carla Caroline ; de Oliveira Ribeiro, Rafael Antonio ; Leite, Maria Luisa ; Soares, Diana Gabriela ; Hebling, Josimeri ; Briso, Andre Luiz Fraga ; de Souza Costa, Carlos Alberto
Total Authors: 8
Document type: Journal article
Source: CLINICAL ORAL INVESTIGATIONS; v. 26, n. 5, p. 10-pg., 2022-02-23.

Objectives Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H2O2), applied for different time intervals, on enamel coated or not with polymeric biomaterials. Materials and methods Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H2O2, according to the following groups: G1-negative control (NC, without treatment); G2, G3, and G4-BG-35%H2O2 applied for 3 x 15, 2 x 15, and 15 min; G5, G6, and G7-BG-35%H2O2 applied on enamel coated with NSc and PrCa for 3 x 15; 2 x 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H2O2 diffused and esthetic efficacy (Delta E/Delta WI) of the dental tissues were also analyzed (ANOVA/Tukey; p < 0.05). Results Only G7 was similar to G1 regarding VB (p > 0.05). The lowest value of H2O2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 (p < 0.05). Despite G5 showing the greatest Delta E regarding other groups (p < 0.05), the esthetic efficacy observed in G7 was similar to G2 (p > 0.05). Delta WI indicated a greater bleaching effect for groups G5, G6, and G7 (p < 0.05). Conclusion Coating the dental enamel with polymeric biomaterials reduced the time and the cytotoxicity of BG-35%H2O2. Clinical significance. Coating the dental enamel with polymeric biomaterials allows safer and faster BG-35%H2O2 application. (AU)

FAPESP's process: 17/22739-9 - Development and analysis of a bilayer scaffold for pulp-dentin regeneration
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants