Advanced search
Start date

Optimizing studies of phagocytic activity by flowsight cytometry in amphibians

Full text
Monteiro Titon, Stefanny Christie ; Assis, Vania Regina ; Titon Junior, Braz ; Kinker, Gabriela Sarti ; Teixeira de Queiroz Hazarbassanov, Nicolle Gilda ; Lima, Alan Siqueira ; Massoco, Cristina de Oliveira ; Fernandes, Pedro A. ; Gomes, Fernando Ribeiro ; Markus, Regina P.
Total Authors: 10
Document type: Journal article
Source: South American Journal of Herpetology; v. 23, n. 1, p. 9-pg., 2022-04-01.

Phagocytosis is a primary and highly conserved mechanism for clearing the extracellular milieu from pathogens and debris. In amphibians, the lack of antibodies for characterizing the different phenotypes of phagocytic cells has impaired the study of the phagocytic process. We used conventional and flowsight cytometry to determine immune cells' phagocytic activity from the blood and peritoneum of toads by in vitro and in vivo assays. Macrophage-like and neutrophil-like cells were clustered and analyzed according to cell morphology and the number of internalized zymosan particles by flowsight cytometry. We identified peritoneal and blood phagocytes (macrophage-like/monocyte-like and neutrophil-like) and lymphocyte-like cells. Besides, we observed monocyte-like/macrophage-like and neutrophil-like cells engulfing up to seven zymosan particles. Assessing the phagocytic activity from blood and peritoneal phagocytes using in vitro and in vivo assays brings better insights into phagocytosis in amphibian immune cells from distinct body compartments and approaches. Moreover, it is worth highlighting the importance of morphologically identifying the cells and evaluating the number of internalized particles by flowsight cytometry, a valuable asset to further explore phagocytosis and other cellular processes in amphibians under field and laboratory conditions. (AU)

FAPESP's process: 13/00900-1 - Comparative study of the relationships between immunocompetence and plasmatic levels of gonadal and inter-renal steroids in toads
Grantee:Fernando Ribeiro Gomes
Support Opportunities: Regular Research Grants
FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
Grantee:Carlos Arturo Navas Iannini
Support Opportunities: Research Program on Global Climate Change - Thematic Grants
FAPESP's process: 13/13691-1 - Immune-pineal axis: time-niology integrated to surveillance and defense
Grantee:Regina Pekelmann Markus
Support Opportunities: Research Projects - Thematic Grants