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New antituberculosis agents\' candidates: design and synthesis of phosphopantetheine adenylyltransferase inhibitors

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Marina Candido Primi
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ)
Defense date:
Examining board members:
Elizabeth Igne Ferreira; Marcio Vinícius Bertacine Dias
Advisor: Elizabeth Igne Ferreira; Carlos Maurício Rabello de Sant'Anna

Tuberculosis is one of the major causes of death by infection worldwide. In 2015, 10.4 thousand new cases of the disease were registered. The tuberculosis\' causing agent Mycobacterium tuberculosis presents high levels of resistance for the available chemotherapy. Thereof, exploit new M. tuberculosis targets is of utmost importance to overcome drug resistant tuberculosis. In this sense, the enzyme phosphopantetheine adenylyltransferase (PPAT) generates scientific interest since it displays a regulatory role in the M. tuberculosis coenzyme A (CoA) biosynthesis. Therefore, the purpose of the present study was the development of M. tuberculosis PPAT (MtPPAT) inhibitors. Initially, 50 potentially MtPPAT inhibitors were designed based on MtPPAT\'s substrate and the enzyme\'s active site. After preliminary results, more 28 compounds were designed. Docking simulations were performed with the 78 compounds synthesized, leading to the prediction of the interaction between the proposed inhibitors and MtPPAT active site. Latelly, semi-empirical calculations were performed with the most promising compounds. These calculations were carried out to obtain information about the enthalpy interactions between compounds and MtPPAT active site. Computational studies led to the selection of the most promising inhibitors. Those compounds and some of their fragments were synthesized, purified, and characterized. The synthesized compounds had their in vitro microbiological activity and cytotoxicity evaluated. Some of the synthesized compounds showed activity against the Mtb sensitive and resistant strains in micromolar range. Besides that, the active compounds were not considered cytotoxic. To validate the potential inhibitors\' design and evaluate their capacity to inhibit MtPPAT, the enzyme was produced and purified. MtPPAT inhibitory assays were performed, leading to the first inhibitors of the enzyme, with activity in micromolar range, validating the target. (AU)

FAPESP's process: 13/15947-3 - New antituberculosis agents candidates: design and synthesis of phosphopantetheine adenylyltransferase inhibitors
Grantee:Marina Candido Primi
Support Opportunities: Scholarships in Brazil - Doctorate