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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagass

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Author(s):
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Kuniyoshi, Tais Mayumi [1] ; Nobrega Mendonca, Carlos Miguel [1] ; Vieira, Viviane Borges [1] ; Robl, Diogo [2] ; Gombossy de Melo Franco, Bernadette Dora [3] ; Todorov, Svetoslav Dimitrov [4, 5] ; Tome, Elisabetta [6] ; O'Connor, Paula Mary [7, 8] ; Converti, Attilio [9] ; Araujo, Welington Luiz [10] ; Silva Palmeiras Vasconcellos, Livia Paula [11] ; Varani, Alessandro de Mello [12] ; Cotter, Paul David [7, 8, 13] ; Rabelo, Sarita Candida [14] ; de Souza Oliveira, Ricardo Pinheiro [1]
Total Authors: 15
Affiliation:
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[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil
[2] Fed Univ Santa Catarina UFSC, Dept Microbiol Immunol & Parasitol, BR-88040900 Florianopolis, SC - Brazil
[3] Univ Sao Paulo, FoRC Food Res Ctr, Sch Pharmaceut Sci, Food & Expt Nutr Dept, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci, Food & Expt Nutr Dept, BR-05508000 Sao Paulo - Brazil
[5] Handong Global Univ, ProBacLab, Adv Convergence, Pohang 37554, Gyeongbuk - South Korea
[6] Univ Cent Venezuela, Inst Ciencias & Tecnol Alimentos, Escuela Biol, Apartado 47-097, Caracas 1041A - Venezuela
[7] TEAGASC, Food Biosci Dept, Food Res Ctr, Moorepk, Fermoy T12 YN60, Cork - Ireland
[8] Univ Coll Cork, APC Microbiome Ireland, Cork T12YT20 - Ireland
[9] Genoa Univ, Dept Civil Chem & Environm Engn, I-16145 Genoa - Italy
[10] Univ Sao Paulo, Microbiol Dept, Biomed Sci Inst, BR-05508900 Sao Paulo - Brazil
[11] Natl Res Ctr Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas - Brazil
[12] Sao Paulo State Univ, Fac Ciencias Agr & Vet, Sch Agr & Vet Sci, Dept Technol, BR-14884000 Jaboticabal, SP - Brazil
[13] Univ Coll Cork, Sch Microbiol, Cork T12YT20 - Ireland
[14] Sao Paulo State Univ, Coll Agr Sci, Dept Bioproc & Biotechnol, BR-18610034 Sao Paulo - Brazil
Total Affiliations: 14
Document type: Journal article
Source: Bioresource Technology; v. 338, OCT 2021.
Web of Science Citations: 2
Abstract

Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg.L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production. (AU)

FAPESP's process: 13/07914-8 - FoRC - Food Research Center
Grantee:Bernadette Dora Gombossy de Melo Franco
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 18/04385-8 - Construction of a pediocin-producing recombinant lineage from agroindustrial waste
Grantee:Ricardo Pinheiro de Souza Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 15/24777-0 - Optimization of pediocin A production in Pediococcus pentosaceus ATCC 43200
Grantee:Taís Mayumi Kuniyoshi
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
Grantee:Ricardo Pinheiro de Souza Oliveira
Support Opportunities: Research Projects - Thematic Grants