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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Building an embryo: An auxin gene toolkit for zygotic and somatic embryogenesis in Brazilian pin

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Elbl, Paula M. [1] ; de Souza, Diego T. [2, 1] ; Rosado, Daniele [3] ; de Oliveira, Leandro F. [1] ; Navarro, V, Bruno ; Matioli, Sergio R. [4] ; Floh, Eny I. S. [5]
Total Authors: 7
[1] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Rua Matao 277, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Math & Stat, Dept Comp Sci, Sao Paulo, SP - Brazil
[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 - USA
[4] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, Sao Paulo, SP - Brazil
[5] Navarro, Bruno, V, Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Rua Matao 277, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Gene; v. 817, APR 5 2022.
Web of Science Citations: 0

Many studies in the model species Arabidopsis thaliana characterized genes involved in embryo formation. However, much remains to be learned about the portfolio of genes that are involved in signal transduction and transcriptional regulation during plant embryo development in other species, particularly in an evolutionary context, especially considering that some genes involved in embryo patterning are not exclusive of land plants. This study, used a combination of domain architecture phylostratigraphy and phylogenetic reconstruction to investigate the evolutionary history of embryo patterning and auxin metabolism (EPAM) genes in Viridiplantae. This approach shed light on the co-optation of auxin metabolism and other molecular mechanisms that contributed to the radiation of land plants, and specifically to embryo formation. These results have potential to assist conservation programs, by directing the development of tools for obtaining somatic embryos. In this context, we employed this methodology with critically endangered and non-model species Araucaria angustifolia, the Brazilian pine, which is current focus of conservation efforts using somatic embryogenesis. So far, this approach had little success since somatic embryos fail to completely develop. By profiling the expression of genes that we identified as necessary for the emergence of land-plant embryos, we found striking differences between zygotic and somatic embryos that might explain the developmental arrest and be used to improve A. angustifolia somatic culture. (AU)

FAPESP's process: 18/08215-0 - Study of post-transcriptional gene regulation processes in the somatic embryogenesis in Araucaria angustifolia
Grantee:Leandro Francisco de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/17541-2 - From stress to embryos: molecular and physiological mechanisms associated to somatic embryogenesis
Grantee:Eny Iochevet Segal Floh
Support type: Regular Research Grants
FAPESP's process: 17/25680-5 - Development and integration of computational tools for Piper species transcriptome analysis
Grantee:Diego Trindade de Souza
Support type: Scholarships in Brazil - Technical Training Program - Technical Training