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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

henothiazinium dyes for photodynamic treatment present lower environmental risk compared to a formulation of trifloxystrobin and tebuconazol

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Andrade, Gabriela Carvalho [1] ; Pereira Brancini, Guilherme Thomaz [1] ; Abe, Flavia Renata [1] ; de Oliveira, Danielle Palma [1, 2] ; Nicolella, Heloiza Diniz [3] ; Tavares, Denise Crispim [3] ; Ditondo Micas, Andre Fernando [4] ; Savazzi, Eduardo Angelino [4] ; Silva-Junior, Geraldo Jose [5] ; Wainwright, Mark [6] ; Leite Braga, Gilberto Ubida [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Chem, Natl Inst Alternat Technol Detect Toxicol Evaluat, BR-14800060 Araraquara, SP - Brazil
[3] Univ Franca, Lab Mutagenesis, BR-14404600 Franca, SP - Brazil
[4] Companhia Ambiental Estado Sao Paulo CETESB, Div Lab Ribeirao Preto, BR-14096350 Ribeirao Preto, SP - Brazil
[5] Fundecitrus, Fund Citrus Protect, BR-14807040 Araraquara, SP - Brazil
[6] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool L3 3AF, Merseyside - England
Total Affiliations: 6
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 226, JAN 2022.
Web of Science Citations: 0
Abstract

The widespread use of conventional chemical antifungal agents has led to worldwide concern regarding the selection of resistant isolates. In this scenario, antimicrobial photodynamic treatment (APDT) has emerged as a promising alternative to overcome this issue. The technique is based on the use of a photosensitizer (PS) and light in the presence of molecular oxygen. Under these conditions, the PS generates reactive oxygen species which damage the biomolecules of the target organism leading to cell death. The great potential of APDT against plantpathogenic fungi has already been reported both in vitro and in planta, indicating this control measure has the potential to be widely used in crop plants. However, there is a lack of studies on environmental risk with ecotoxicological assessment of PSs used in APDT. Therefore, this study aimed to evaluate the environmental toxicity of four phenothiazinium PSs: i) methylene blue (MB), ii) new methylene blue N (NMBN), iii) toluidine blue O (TBO), and iv) dimethylmethylene blue (DMMB) and also of the commercial antifungal NATIVO (R), a mixture of trifloxystrobin and tebuconazole. The experiments were performed with Daphnia similis neonates and zebrafish embryos. Our results showed that the PSs tested had different levels of toxicity, with MB being the less toxic and DMMB being the most. Nonetheless, the environmental toxicity of these PSs were lower when compared to that of NATIVO (R). Furthermore, estimates of bioconcentration and of biotransformation half-life indicated that the PSs are environmentally safer than NATIVO (R). Taken together, our results show that the toxicity associated with phenothiazinium PSs would not constitute an impediment to their use in APDT. Therefore, APDT is a promising approach to control plant-pathogenic fungi with reduced risk for selecting resistant isolates and lower environmental impacts when compared to commonly used antifungal agents. (AU)

FAPESP's process: 16/24269-7 - Analysis of the effects of manool, a diterpene of Salvia officinalis, on cell and genomic integrity.
Grantee:Denise Crispim Tavares Barbosa
Support Opportunities: Regular Research Grants
FAPESP's process: 18/24298-2 - USE OF MULTI-BIOMARkER STRATEGY IN ZEBRAFISH AS A PLATFORM TO ESTIMATE NEUROCOMPORTAMENTAL EFFECTS INDUCED BY FLAME RETARDANTS
Grantee:Danielle Palma de Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 18/06945-0 - Zebrafish as a model to predict effects of multiple environmental stressors: using a multi-biomarker approach
Grantee:Flavia Renata Abe
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
Grantee:Maria Valnice Boldrin
Support Opportunities: Research Projects - Thematic Grants