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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cervicovaginal levels of human beta defensins during bacterial vaginosis

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Noda-Nicolau, Nathalia Mayumi [1] ; Silva, Mariana de Castro [1] ; Cosi Bento, Giovana Fernanda [1] ; Baptista Ferreira, Jeniffer Sena [1] ; Novak, Juliano [1] ; Pessoa Morales, Julia Andrade [1] ; Tronco, Julia Abbade [1] ; Bolpetti, Aline Nascimento [1] ; Silva Pinto, Gabriel Vitor [1] ; Polettini, Jossimara [2] ; Marconi, Camila [1, 3] ; da Silva, Marcia Guimaraes [1]
Total Authors: 12
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Pathol, Sao Paulo - Brazil
[2] Univ Fed Fronteira Sul UFFS, Med Sch, Grad Program Biomed Sci, Passo Fundo, RS - Brazil
[3] Univ Fed Parana UFPR, Dept Basic Pathol, Setor Ciencias Biol, Curitiba, Parana - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 16, n. 12 DEC 2 2021.
Web of Science Citations: 0

Aims To compare the cervicovaginal levels of human beta defensin (hBD)-1, 2 and 3 of women according to the status of Nugent-defined bacterial vaginosis (BV). Methods A total of 634 women of reproductive age were included in the study. Participants were equally distributed in two groups: according to the classification of vaginal smears according to Nugent criteria in normal (scores 0 to 3) and BV (scores >= 7). Cervicovaginal fluid samples were used for measurements of hBDs1, 2 and 3 levels by enzyme-linked immunosorbent assay (ELISA). Levels of each hBD were compared between the two study groups using Mann-Whitney test, with p-value <0.05 considered as significant. Odds ratio (OR) and 95% confidence interval (95% CI) were calculated for sociodemographic variables and hBD1-3 levels associated with BV a multivariable analysis. Correlation between Nugent score and measured levels of hBDs1-3 were calculated using Spearman's test. Results Cervicovaginal fluids from women with BV showed lower levels of hBD-1 {[}median 2,400.00 pg/mL (0-27,800.00); p<0.0001], hBD-2 {[}5,600.00 pg/mL (0-45,800.00); p<0.0001] and hBD-3 {[}1,600.00 pg/mL (0-81,700.00); p = 0.012] when compared to optimal microbiota {[}hBD-1: {[}median 3,400.00 pg/mL (0-35,600.00), hBD-2: 12,300.00 pg/mL (0-95,300.00) and hBD-3: 3,000.00 pg/mL (0-64,300.00), respectively]. Multivariable analysis showed that lower levels of hBD-1 (OR: 2.05; 95% CI: 1.46-2.87), hBD-2 (OR: 1.85; 95% CI: 1.32-2.60) and hBD-3 (OR: 1.90; 95% CI: 1.37-2.64) were independently associated BV. Significant negative correlations were observed between Nugent scores and cervicovaginal levels of hBD-1 (Spearman's rho = -0.2118; p = 0.0001) and hBD-2 ({*}Spearman's rho = -0.2117; p = 0.0001). Conclusions Bacterial vaginosis is associated with lower cervicovaginal levels of hBDs1-3 in reproductive-aged women. (AU)

FAPESP's process: 12/16800-3 - Characterization of the vaginal microbiome of Brazilian women in reproductive age
Grantee:Márcia Guimarães da Silva
Support Opportunities: Regular Research Grants