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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes

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Valerio, Hellen Paula [1] ; Ravagnani, Felipe Gustavo [1] ; Ronsein, Graziella Eliza [1] ; Di Mascio, Paolo [1]
Total Authors: 4
[1] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 11, n. 1 DEC 2 2021.
Web of Science Citations: 0

Epidermal photoaging contributes to skin fragility over time and it is a risk factor for skin cancer. Photoaging has been associated for a long time with exposure to Ultraviolet-A (UVA) light, the predominant component of the solar ultraviolet radiation. While the cellular mechanisms underlying UVA-induced photoaging in the dermis have been well characterized, UVA's action on the epidermis remains elusive. Here, proteomic analysis was conducted to derive the cellular responses induced by an environmentally relevant dose of UVA in primary human keratinocytes. We also investigated the effects of UVA on non-transformed immortalized keratinocytes (HaCaT cells), bearing potentially oncogenic mutations. We showed that UVA induces proteome remodeling and senescence in primary keratinocytes, eliciting potent antioxidant and pro-inflammatory responses. Additionally, we showed that UVA modulates the secretory phenotype of these cells to the extent of inducing paracrine oxidative stress and immune system activation in pre-malignant keratinocytes. These observations offer insights into the cellular mechanisms by which UVA drives photoaging in the skin. (AU)

FAPESP's process: 15/07768-7 - Programmed cell death photoinduced mechanisms and different gene expression profiles
Grantee:Felipe Gustavo Ravagnani
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/12663-1 - Singlet molecular oxygen and peroxides in chemical biology
Grantee:Paolo Di Mascio
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/11430-4 - Proteome changes and DNA damage caused by photoinduced oxidative stress in cells
Grantee:Hellen Paula Valerio
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/00696-3 - Proteomic as a tool to understand HDL function and composition
Grantee:Graziella Eliza Ronsein
Support Opportunities: Research Grants - Young Investigators Grants