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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maternal Separation Stress Affects Voluntary Ethanol Intake in a Sex Dependent Manner

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Bertagna, Natalia Bonetti [1] ; Favoretto, Cristiane Aparecida [1] ; Rodolpho, Ben Tagami [1] ; Palombo, Paola [1] ; Yokoyama, Thais Suemi [1] ; Righi, Thamires [1] ; Loss, Cassio Morais [1, 2] ; Leao, Rodrigo Molini [3] ; Miguel, Tarciso Tadeu [3] ; Cruz, Fabio Cardoso [1]
Total Authors: 10
[1] Univ Fed Sao Paulo, Dept Pharmacol, Mol & Behav Neurosci Lab, Sao Paulo - Brazil
[2] Natl Council Sci & Technol Dev CNPq, FAPESP, CAPES, Natl Inst Translat Med INCT TM, Ribeirao Preto - Brazil
[3] Univ Fed Uberlandia, Biomed Sci Inst, Dept Pharmacol, Pharmacol Lab, Uberlandia, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN PHYSIOLOGY; v. 12, DEC 7 2021.
Web of Science Citations: 0

Maternal separation (MS) stress is a predictive animal model for evaluating the effects of early stress exposure on alcohol use disorders (AUD). The extended amygdala (AMY) is a complex circuit involved in both stress- and ethanol-related responses. We hypothesized that MS stress may increase ethanol consumption in adulthood, as well as augment neuronal activity in extended AMY, in a sex-dependent manner. We aimed to investigate the influence of MS stress on the ethanol consumption of male and female mice, and the involvement of extended amygdala sub-nuclei in this process. The C57BL/6J pups were subjected to 180min of MS, from postnatal day (PND) 1 to 14. The control group was left undisturbed. On PND 45, mice (n=28) in cages were exposed to a bottle containing 20% ethanol (w/v) for 4h during the dark period of the light-dark cycle, for 3weeks. Afterward, mice underwent ethanol self-administration training in operant chambers under fixed ratio (FR) schedule. Then, subjects were tested under 2h sessions of a progressive-ratio (PR) schedule of reinforcement (the last ratio achieved was considered the breaking point), and at the end, a 4h session of FR schedule (binge-intake). An immunohistochemistry assay for Fos protein was performed in Nucleus Accumbens (NAcc), Bed Nucleus of Stria Terminalis (BNST), and AMY. Our results showed that in the third week of training, the female MS group consumed more ethanol than the respective control group. The MS group presented increased breakpoint parameters. Female control group and male MS group were more resistant to bitter quinine taste. Increased Fos-immunoreactive neurons (Fos-IR) were observed in the central nucleus of AMY, but not in NAcc nor BNST in male maternal-separated mice. Maternal separation stress may influence ethanol intake in adulthood, and it is dependent on the sex and reinforcement protocol. (AU)

FAPESP's process: 20/04389-3 - Maternal separation stress and behaviors related to alcohol dependence in mice: assessment of CRFergic neurotransmission
Grantee:Natalia Bonetti Bertagna
Support type: Scholarships in Brazil - Doctorate