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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bifidobacterium Strains Present Distinct Effects on the Control of Alveolar Bone Loss in a Periodontitis Experimental Model

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Author(s):
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Shimabukuro, Natali [1, 2] ; Cataruci, Amalia C. de S. [1, 2] ; Ishikawa, Karin H. [1] ; Oliveira, Bruna E. de [1] ; Kawamoto, Dione [1] ; Ando-Suguimoto, Ellen S. [1] ; Albuquerque-Souza, Emmanuel [2] ; Nicoli, Jacques R. [3] ; Ferreira, Caroline M. [4] ; de Lima, Jean [5] ; Bueno, Manuela R. [2] ; da Silva, Leandro B. R. [1] ; Silva, Pedro H. F. [6] ; Messora, Michel R. [6] ; Camara, Niels O. S. [5] ; Simionato, Maria Regina L. [1] ; Mayer, Marcia P. A. [1, 2]
Total Authors: 17
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Div Periodont, Sao Paulo - Brazil
[3] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Belo Horizonte, MG - Brazil
[4] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Pharmaceut Sci, Diadema - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil
[6] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Traumatol & Peri, Ribeirao Preto - Brazil
Total Affiliations: 6
Document type: Journal article
Source: FRONTIERS IN PHARMACOLOGY; v. 12, SEP 24 2021.
Web of Science Citations: 0
Abstract

Periodontitis is an inflammatory disease induced by a dysbiotic oral microbiome. Probiotics of the genus Bifidobacterium may restore the symbiotic microbiome and modulate the immune response, leading to periodontitis control. We evaluated the effect of two strains of Bifidobacterium able to inhibit Porphyromonas gingivalis interaction with host cells and biofilm formation, but with distinct immunomodulatory properties, in a mice periodontitis model. Experimental periodontitis (P+) was induced in C57Bl/6 mice by a microbial consortium of human oral organisms. B. bifidum 162(2A) {[}B+ (1622)] and B. breve 110(1A) {[}B+ (1101)] were orally inoculated for 45 days. Alveolar bone loss and inflammatory response in gingival tissues were determined. The microbial consortium induced alveolar bone loss in positive control (P + B-), as demonstrated by microtomography analysis, although P. gingivalis was undetected in oral biofilms at the end of the experimental period. TNF-alpha and IL-10 serum levels, and Treg and Th17 populations in gingiva of SHAM and P + B- groups did not differ. B. bifidum 162(2A), but not B. breve 110(1A), controlled bone destruction in P+ mice. B. breve 110(1A) upregulated transcription of Il-1 beta, Tnf-alpha, Tlr2, Tlr4, and Nlrp3 in P-B+(1101), which was attenuated by the microbial consortium {[}P + B+(1101)]. All treatments downregulated transcription of Il-17, although treatment with B. breve 110(1A) did not yield such low levels of transcripts as seen for the other groups. B. breve 110(1A) increased Th17 population in gingival tissues {[}P-B+ (1101) and P + B+ (1101)] compared to SHAM and P + B-. Administration of both bifidobacteria resulted in serum IL-10 decreased levels. Our data indicated that the beneficial effect of Bifidobacterium is not a common trait of this genus, since B. breve 110(1A) induced an inflammatory profile in gingival tissues and did not prevent alveolar bone loss. However, the properties of B. bifidum 162(2A) suggest its potential to control periodontitis. (AU)

FAPESP's process: 17/22345-0 - Effect of Bifidobacterium probiotics on the modulation of immune response and alveolar bone loss in an experimental periodontitis model on normoglycemic and diabetic mice
Grantee:Natali Shimabukuro
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/18273-9 - New strategies for the control of periodontitis
Grantee:Marcia Pinto Alves Mayer
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/13156-7 - Direct and immunomodulatory effects of probiotic bacteria on periodontal pathogens
Grantee:Karin Hitomi Ishikawa
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/25171-6 - Prospection of new epitopes with vaccine potential in the control of experimental infection by Paracoccidioides lutzii
Grantee:Leandro Buffoni Roque da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/14687-6 - Evaluation of immunomodulatory potential of probiotics in periodontal disease
Grantee:Emmanuel Albuquerque de Souza
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/16377-7 - EVALUATION OF THE EFFECT OF PROBIOTIC BACTERIA ON THE IMUNE RESPONSE TO A.ACTINOMYCETEMCOMITANS: A STUDY IN GINGIVAL EPITHELIAL CELLS AND IN EXPERIMENTAL MODEL OF INFECTION IN VIVO.
Grantee:Manuela Rocha Bueno
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/13159-6 - Supra and subgingival microbiota diversity analysis and protein profile of saliva and enamel film of individuals with chronic periodontitis and microbial succession
Grantee:Dione Kawamoto
Support type: Scholarships in Brazil - Post-Doctorate