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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Qualea grandiflora Extract on the Expression of MMP-14 and HIF-1 alpha in Cultured Fibroblasts and Preosteoblasts

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Author(s):
Neubern de Oliveira, Gabriela Silva [1] ; Tokuhara, Cintia Kazuko [1] ; de Oliveira, Flavia Amadeu [1] ; Zardin Graeff, Marcia Sirlene [1] ; Khan, Zohaib Nisar [1] ; Saldanha, Luiz Leonardo [2] ; Dokkedal, Anne Ligia [2] ; de Oliveira, Rodrigo Cardoso [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[2] Sao Paulo State Univ, Fac Sci, Dept Biol Sci, Bauru, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Archives of Biology and Technology; v. 64, 2021.
Web of Science Citations: 0
Abstract

Background: Qualea grandiflora (QG) (Vochysiaceae), also known as ``pau-ferro{''}, ``pau-terra{''} or ``pau-de-tucano{''}, is a very common deciduous tree in the Brazilian Cerrado used in traditional medicine to treat inflammations, ulcers, diarrhea, and infections. There are reports in the scientific literature that demonstrate the medicinal effects of the bark and leaf of the QG. However, studies involving this plant are rather imited. Aim of the study: To perform the phytochemical analysis of the QG hydroalcoholic extract (HAE) of leaves, and to investigate it effects on fibroblast and preosteoblasts. Methods: Phytochemical analysis was done by HPLC-DAD. Murine NIH/3T3 fibroblasts and MC3T3-E1 preosteoblasts cell lines (ATCC) were used for the experiments. Cell viability was assessed by the MTT colorimetric assay and the expression of MMP-14 and HIF-1 alpha by immunofluorescence. Results and conclusion: The following compounds were identified by HPLC-DAD, such as quinic acid, ethyl galate, ellagic acid derivatives as O-methylellagic acid O-galloyl, O-methylellagic acid O-deoxyhexoside, galloyl derivatives, flavonol glycoside as kaempferol-O-deoxyhexoside, quercetin-O-deoxyhexoside, myricetin-O-deoxyhexoside and the pentacyclic triterpene arjunglucoside. Cell viability results demonstrated no cytotoxic effects in the studied concentrations. We found in QG HAE some compounds with therapeutic properties that can increase the expression of MMP-14 and HIF-1 alpha, in fibroblasts and preosteoblasts. These data suggest that QG HAE has an action on these two molecules widely involved in physiological conditions, such as collagen remodeling, bone development and growth and pathological processes as HIF signaling in cancer metastasis. (AU)

FAPESP's process: 14/05234-2 - Study of the aqueous extract of leaves of Qualea grandiflora: phytochemical evaluation and effects on the expression of proteins involved in inflammatory processes
Grantee:Rodrigo Cardoso de Oliveira
Support type: Regular Research Grants
FAPESP's process: 14/20656-0 - Evaluation of Qualea grandiflora Mart effect on HIF-1 alpha and MMP-14 modulation in cell lines of pre osteoblasts and fibroblastos
Grantee:Gabriela Silva Neubern de Oliveira
Support type: Scholarships in Brazil - Master